Involvement of vesicle-cytoskeleton interaction in AQP5 trafficking in AQP5-gene-transfected HSG cells.
Involvement of vesicle-cytoskeleton interaction in AQP5 trafficking in AQP5-gene-transfected HSG cells.
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囊泡-细胞骨架相互作用参与 AQP5 基因转染的 HSG 细胞中的 AQP5 运输。
DOI：
10.1006/bbrc.1999.1828
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发表时间：
1999
影响因子：
3.1
通讯作者：
K. Hosoi
中科院分区：
文献类型：
--
作者：
J. Tada;T. Sawa;N. Yamanaka;M. Shono;T. Akamatsu;K. Tsumura;M. Parvin;N. Kanamori;K. Hosoi
将大鼠水通道蛋白5（AQP5）的cDNA转染至HSG（人唾液腺细胞），并通过共聚焦激光显微镜在体外研究其转运机制。 AQP5 向质膜的运输是通过用毒胡萝卜素（一种内质 Ca(2+)-ATP 酶抑制剂）和/或用 A-23187（一种钙离子载体）刺激 AQP5 基因转染的人唾液腺细胞（HSGAQP5 细胞）来诱导的。用微管抑制剂秋水仙碱或长春花碱预处理这些细胞，可阻止毒胡萝卜素或 A-23187 诱导的运输。运输事件并未被微丝抑制剂细胞松弛素 B 完全抑制。这些结果表明，AQP5囊泡向质膜的运输是由细胞内Ca(2+)的增加触发的，并且含有AQP5的囊泡与细胞骨架的相互作用参与了这种运输。
A cDNA of rat aquaporin 5 (AQP5) was used to transfect to HSG (human salivary gland cells), and the trafficking mechanism was studied in vitro by confocal laser microscopy. The trafficking of AQP5 to the plasma membrane was induced by stimulation of AQP5-gene-transfected human salivary gland cells (HSGAQP5 cells) with thapsigargin, an inhibitor of endoplasmic Ca(2+)-ATPase, and or with A-23187, a calcium ionophore. Pretreatment of these cells with colchicine or vinblastine, microtubule inhibitors, prevented the trafficking induced by thapsigargin or A-23187. The trafficking event was not completely inhibited by cytochalasin B, a microfilament inhibitor. These results demonstrate that the trafficking of AQP5 vesicles to the plasma membrane is triggered by an increase in intracellular Ca(2+) and that the interaction of AQP5-containing vesicles with the cytoskeleton is involved in this trafficking.
DOI：
10.1073/pnas.88.24.11110
发表时间：
1991-12-01
影响因子：
11.1
作者：
PRESTON, GM;AGRE, P
通讯作者：
AGRE, P