Interferon-γ-Mediated Natural Killer Cell Activation by an Aqueous Panax ginseng Extract.
Interferon-γ-Mediated Natural Killer Cell Activation by an Aqueous Panax ginseng Extract.
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DOI：
10.1155/2015/603198
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Okumura K
中科院分区：
文献类型：
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作者：
Takeda K;Okumura K
人参提取物用于传统草药，特别是在东亚，但其对自然杀伤（NK）细胞活性的影响尚未完全了解。本研究旨在检测人参提取物对NK细胞的细胞毒活性的影响。我们口服给予野生型（WT）C57 BL/6（B6）和BALB/c小鼠以及重组激活基因2（RAG-2）或干扰素-γ（IFN-γ）缺陷的B6小鼠人参提取物或人参皂苷。然后，我们测试了NK细胞（脾和肝单核细胞）对NK敏感的YAC-1细胞的细胞毒性活性。口服人参水提物可增强WT B6和BALB/c小鼠以及RAG-2缺陷型B6小鼠NK细胞的细胞毒性，但对IFN-γ缺陷型B6小鼠无明显影响。这种作用仅在人参的水提取物中观察到。有趣的是，人参皂苷Rb 1和Rg 1没有增加NK细胞的细胞毒性。这些结果表明，人参水提取物通过IFN-γ依赖性途径增强体内NK细胞活化。
Panax ginseng extracts are used in traditional herbal medicines, particularly in eastern Asia, but their effect on natural killer (NK) cell activity is not completely understood. This study aimed to examine the effects of P. ginseng extracts on the cytotoxic activity of NK cells. We orally administered P. ginseng extracts or ginsenosides to wild-type (WT) C57BL/6 (B6) and BALB/c mice and to B6 mice deficient in either recombination activating gene 2 (RAG-2) or interferon-γ (IFN-γ). We then tested the cytotoxic activity of NK cells (of spleen and liver mononuclear cells) against NK-sensitive YAC-1 cells. Oral administration of P. ginseng aqueous extract augmented the cytotoxicity of NK cells in WT B6 and BALB/c mice and in RAG-2-deficient B6 mice, but not in IFN-γ-deficient B6 mice. This effect was only observed with the aqueous extract of P. ginseng. Interestingly, the ginsenosides Rb1 and Rg1 did not augment NK cell cytotoxicity. These results demonstrated that the aqueous P. ginseng extract augmented NK cell activation in vivo via an IFN-γ-dependent pathway.