Separomics applied to the proteomics and peptidomics of low-abundance proteins: Choice of methods and challenges - A review.
Separomics applied to the proteomics and peptidomics of low-abundance proteins: Choice of methods and challenges - A review.
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DOI：
10.1590/s1415-47572012000200009
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发表时间：
2012-06
影响因子：
2.1
通讯作者：
de Barros EG
中科院分区：
文献类型：
--
作者：
Baracat-Pereira MC;de Oliveira Barbosa M;Magalhães MJ Jr;Carrijo LC;Games PD;Almeida HO;Sena Netto JF;Pereira MR;de Barros EG
蛋白质的富集和分离被认为是蛋白质组学研究中的限制性步骤。鉴定瞬时表达的蛋白质、低丰度的蛋白质以及数据库中未描述的天然肽仍然是一个巨大的挑战。植物提取物通常是复杂的，并且污染物干扰参与重要生理过程的蛋白质的鉴定，例如植物对病原体的防御。本文综述了分离组学在植物低丰度蛋白质和多肽鉴定中的应用，特别是在受病原菌侵染的植物中的应用。分离组学被描述为用于蛋白质组学的蛋白质分子分离的一组方法学策略。已经使用了几种工具来从样品中去除高度丰富的蛋白质以及非蛋白质污染物。色谱技术的使用，蛋白质组的分区成亚蛋白质组，并努力分离蛋白质在其天然形式允许的稀有蛋白质的分离和鉴定参与不同的过程。
The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes.