Immunoregulatory effect of bifidobacteria strains in porcine intestinal epithelial cells through modulation of ubiquitin-editing enzyme A20 expression.
Immunoregulatory effect of bifidobacteria strains in porcine intestinal epithelial cells through modulation of ubiquitin-editing enzyme A20 expression.
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DOI：
10.1371/journal.pone.0059259
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kitazawa H
中科院分区：
文献类型：
--
作者：
Tomosada Y;Villena J;Murata K;Chiba E;Shimazu T;Aso H;Iwabuchi N;Xiao JZ;Saito T;Kitazawa H
我们以前表明，评价乳酸菌在猪肠上皮细胞（PIE）中的抗炎活性对于选择潜在的免疫生物菌株是有用的。本研究的目的是：i）选择有益地调节PIE细胞中Toll样受体（TLR）-4触发的炎症反应的潜在免疫调节性双歧杆菌; ii）通过评估TLR 2和TLR负调节剂在促炎细胞因子产生和有丝分裂原活化的调节中的作用，深入了解免疫生物素抗炎作用中涉及的分子机制。活化蛋白激酶（MAPK）和核因子-κ B（NF-κ B）途径。 长双歧杆菌BB 536和B.短M-16V菌株显著下调了热灭活的产肠毒素大肠杆菌攻击的PIE细胞中白细胞介素（IL）-8、单核细胞趋化蛋白（MCP）-1和IL-6的水平。此外，BB536和M-16 V菌株通过调节NF-κ B和MAPK通路来减弱促炎反应。此外，我们的研究结果为泛素编辑酶A20在PIE细胞中免疫生物素细菌的抗炎作用中的关键作用提供了证据。我们展示了有关免疫生物素抗炎作用机制的新数据。具有免疫调节能力的几种菌株使用共同的机制来诱导PIE细胞中的耐受性。免疫调节菌株与TLR2相互作用，上调PIE细胞中A20的表达，并通过减少MAPK和NF-κ B通路的激活以及促炎细胞因子的产生来有利地调节随后的TLR4激活。我们还表明，TLR2激活和A20诱导的组合可用作筛选和选择潜在的免疫调节性双歧杆菌菌株的生物标志物。
We previously showed that evaluation of anti-inflammatory activities of lactic acid bacteria in porcine intestinal epithelial (PIE) cells is useful for selecting potentially immunobiotic strains. The aims of the present study were: i) to select potentially immunomodulatory bifidobacteria that beneficially modulate the Toll-like receptor (TLR)-4-triggered inflammatory response in PIE cells and; ii) to gain insight into the molecular mechanisms involved in the anti-inflammatory effect of immunobiotics by evaluating the role of TLR2 and TLR negative regulators in the modulation of proinflammatory cytokine production and activation of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways in PIE cells. Bifidobacteria longum BB536 and B. breve M-16V strains significantly downregulated levels of interleukin (IL)-8, monocyte chemotactic protein (MCP)-1 and IL-6 in PIE cells challenged with heat-killed enterotoxigenic Escherichia coli. Moreover, BB536 and M-16V strains attenuated the proinflammatory response by modulating the NF-κB and MAPK pathways. In addition, our findings provide evidence for a key role for the ubiquitin-editing enzyme A20 in the anti-inflammatory effect of immunobiotic bifidobacteria in PIE cells. We show new data regarding the mechanism involved in the anti-inflammatory effect of immunobiotics. Several strains with immunoregulatory capabilities used a common mechanism to induce tolerance in PIE cells. Immunoregulatory strains interacted with TLR2, upregulated the expression of A20 in PIE cells, and beneficially modulated the subsequent TLR4 activation by reducing the activation of MAPK and NF-κB pathways and the production of proinflammatory cytokines. We also show that the combination of TLR2 activation and A20 induction can be used as biomarkers to screen and select potential immunoregulatory bifidobacteria strains.
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DOI：
10.1152/ajpgi.00531.2006
发表时间：
2007-06-01
影响因子：
4.5
作者：
Shibolet, Oren;Podolsky, Daniel K.
通讯作者：
Podolsky, Daniel K.
DOI：
10.1073/pnas.1000092107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Wells, Jerry M.;Rossi, Oriana;van Baarlen, Peter
通讯作者：
van Baarlen, Peter
影响因子：
56.9
作者：
Lee, EG;Boone, DL;Ma, A
通讯作者：
Ma, A
DOI：
10.1084/jem.20071108
发表时间：
2008-02-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Turer EE;Tavares RM;Mortier E;Hitotsumatsu O;Advincula R;Lee B;Shifrin N;Malynn BA;Ma A
通讯作者：
Ma A
影响因子：
30.5
作者：
Boone, DL;Turer, EE;Ma, A
通讯作者：
Ma, A