Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer.
Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer.
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DOI：
10.1126/science.aal5240
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发表时间：
2017-12-15
期刊：
影响因子：
--
通讯作者：
Meyerson M
中科院分区：
文献类型：
--
作者：
Bullman S;Pedamallu CS;Sicinska E;Clancy TE;Zhang X;Cai D;Neuberg D;Huang K;Guevara F;Nelson T;Chipashvili O;Hagan T;Walker M;Ramachandran A;Diosdado B;Serna G;Mulet N;Landolfi S;Ramon Y Cajal S;Fasani R;Aguirre AJ;Ng K;Élez E;Ogino S;Tabernero J;Fuchs CS;Hahn WC;Nuciforo P;Meyerson M
结直肠癌由恶性细胞、非转化细胞和微生物的复杂混合物组成。具核梭杆菌是结直肠癌组织中最常见的细菌物种之一。在这里，我们表明梭杆菌及其相关微生物组（包括拟杆菌属、月形单胞菌属和普雷沃氏菌属）在人类结直肠癌中的定殖在远端转移中得以维持，证明了成对的原发性和转移性肿瘤之间的微生物组稳定性。原位杂交分析显示，梭杆菌主要与转移灶中的癌细胞相关。发现人原发性结直肠腺癌的小鼠异种移植物通过连续传代保留了活的梭杆菌及其相关微生物组。用抗生素甲硝唑治疗携带结肠癌异种移植物的小鼠减少了梭杆菌负荷、癌细胞增殖和总体肿瘤生长。这些观察结果为进一步研究抗菌干预作为梭菌相关结直肠癌患者的潜在治疗提供了依据。
Colorectal cancers comprise a complex mixture of malignant cells, non-transformed cells, and microorganisms. Fusobacterium nucleatum is among the most prevalent bacterial species in colorectal cancer tissues. Here we show that colonization of human colorectal cancers with Fusobacterium and its associated microbiome, —including Bacteroides, Selenomonas, and Prevotella species, —is maintained in distal metastases, demonstrating microbiome stability between paired primary and -metastatic tumors. In situ hybridization analysis revealed that Fusobacterium is predominantly associated with cancer cells in the metastatic lesions. Mouse xenografts of human primary colorectal adenocarcinomas were found to retain viable Fusobacterium and its associated microbiome through successive passages. Treatment of mice bearing a colon cancer xenograft with the antibiotic metronidazole reduced Fusobacterium load, cancer cell proliferation, and overall tumor growth. These observations argue for further investigation of antimicrobial interventions as a potential treatment for patients with Fusobacterium-associated colorectal cancer.
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影响因子：
24.5
作者：
Mima K;Nishihara R;Qian ZR;Cao Y;Sukawa Y;Nowak JA;Yang J;Dou R;Masugi Y;Song M;Kostic AD;Giannakis M;Bullman S;Milner DA;Baba H;Giovannucci EL;Garraway LA;Freeman GJ;Dranoff G;Garrett WS;Huttenhower C;Meyerson M;Meyerhardt JA;Chan AT;Fuchs CS;Ogino S
通讯作者：
Ogino S
结肠菌群和大肠癌的分子特征中的梭杆菌。
影响因子：
11.2
作者：
Tahara T;Yamamoto E;Suzuki H;Maruyama R;Chung W;Garriga J;Jelinek J;Yamano HO;Sugai T;An B;Shureiqi I;Toyota M;Kondo Y;Estécio MR;Issa JP
通讯作者：
Issa JP
影响因子：
--
作者：
Yu YN;Yu TC;Zhao HJ;Sun TT;Chen HM;Chen HY;An HF;Weng YR;Yu J;Li M;Qin WX;Ma X;Shen N;Hong J;Fang JY
通讯作者：
Fang JY
影响因子：
64.5
作者：
Cancer Genome Atlas Research Network. Electronic address: wheeler@bcm.edu;Cancer Genome Atlas Research Network
通讯作者：
Cancer Genome Atlas Research Network
影响因子：
11.2
作者：
Tomkovich S;Yang Y;Winglee K;Gauthier J;Mühlbauer M;Sun X;Mohamadzadeh M;Liu X;Martin P;Wang GP;Oswald E;Fodor AA;Jobin C
通讯作者：
Jobin C