Chronic bacterial rhinosinusitis - Description of a mouse model
Chronic bacterial rhinosinusitis - Description of a mouse model
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DOI：
10.1001/archotol.127.6.657
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发表时间：
2001-06-01
影响因子：
--
通讯作者：
Chole, RA
中科院分区：
文献类型：
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作者：
Jacob, A;Faddis, BT;Chole, RA
目的：研究正常小鼠鼻窦解剖结构并建立慢性细菌性鼻窦炎小鼠模型。设计：解剖学、组织学和病理生理学研究，显示正常小鼠鼻窦解剖结构和手术产生的单侧鼻窦炎症。受试者：21只6周龄雄性C57 BL/6小鼠。干预措施：使用Merocel鼻腔填塞进行单侧上颌窦口阻塞的动物，仅单侧脆弱类杆菌接种的动物，在4周时检查具有窦口阻塞和细菌接种的动物的慢性鼻窦炎的组织学证据。实验干预措施进行了比较，与对侧对照鼻窦内的每只动物和正常和假手术controls.Results：正常小鼠鼻旁窦包括上颌窦，筛窦气细胞，和nervatory型上皮。在实验动物中，对上颌窦外侧壁、鼻中隔和上颌窦的上级鼻甲进行组织学检查。在仅用Merocel或Merocel加细菌接种的动物的实验鼻窦中存在上皮增厚和混乱、杯状细胞增生、炎性浸润和鼻窦纤维化。Merocel和细菌的变化比单独使用Merocel的变化更显著。假手术对照组和单独接种细菌的鼻窦没有显着不同的鼻窦normal animals.Conclusion：单侧上颌窦口阻塞使用Merocel鼻腔填塞沿着与B fragilis接种结果在一个持久的，局部的细菌性鼻窦炎小鼠。
Objectives: To survey normal murine sinonasal anatomy and to create a mouse model for chronic bacterial rhinosinusitis.Design: Anatomic, histologic, and pathophysiologic study displaying normal murine sinonasal anatomy and surgically created unilateral sinonasal inflammation.Subjects: Twenty-one 6-week-old, male C57BL/6 mice.Interventions: Animals that underwent unilateral maxillary sinus ostial obstruction using Merocel nasal packing, animals with unilateral Bacteroides fragilis inoculation alone, and animals with both ostial obstruction and bacterial inoculation were examined at 4 weeks for histologic evidence of chronic sinonasal inflammation. Experimental interventions were compared with contralateral control sinuses within each animal and with normal and sham-operated controls.Results: Normal mouse paranasal sinuses include maxillary sinuses, ethmoid air cells, and respiratory-type epithelium. In experimental animals, the lateral maxillary sinus wall, nasal septum, and superior turbinelle of the maxillary sinus were examined histologically. Epithelial thickening and disarray, goblet cell hyperplasia, inflammatory infiltrates, and sinonasal fibrosis were present in the experimental sinuses of animals packed with Merocel alone or Merocel with bacterial inoculation. Changes seen with Merocel and bacteria were more dramatic than those with Merocel alone. Sham-operated controls and sinuses inoculated with bacteria alone did not differ significantly from the sinuses of normal animals.Conclusion: Unilateral maxillary sinus ostial obstruction using Merocel nasal packing along with B fragilis inoculation results in a persistent, localized bacterial rhinosinusitis in mice.