Fabrication and characterization of porous polycaprolactone scaffold via extrusion-based cryogenic 3D printing for tissue engineering
Fabrication and characterization of porous polycaprolactone scaffold via extrusion-based cryogenic 3D printing for tissue engineering
复制标题
通过基于挤出的低温 3D 打印用于组织工程的多孔聚己内酯支架的制造和表征
DOI：
10.1016/j.matdes.2019.107946
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发表时间：
2019-10-15
影响因子：
8.4
通讯作者：
Li, Wenchao
中科院分区：
文献类型：
--
作者：
Zhang, Wancheng;Ullah, Ismat;Li, Wenchao
早期关于3D多孔PCL支架用于组织工程应用的报道被一些限制所掩盖，如额外的模具成本，相对较低的效率，以及缺乏过程控制。本研究采用挤压低温3D打印（ECP）（-20℃）和随后的冷冻干燥相结合的方法，方便地制备了具有高孔隙度和保真度的聚己内酯（PCL）支架。冻干引起支架沿X、Y、z轴不同程度的收缩。CP600、CP800和CP1000的孔隙率分别为64.0 +/- 1.2%、70.1 +/- 1.3%和74.3 +/- 0.6%。对制备的支架进行了各种结构特征的表征，并与传统的基于挤出的熔融3D打印（EMP）制备的支架进行了比较。ECP支架的PCL结晶度（57.1 +/- 2.2%）低于EMP支架（69.8 +/- 1.3%）。ECP支架具有较高的碱性降解性能，但压缩性能较低。ECP支架可促进MCT3T-E1细胞在多孔细丝上的粘附和增殖。总之，这些特点证明印刷PCL支架适合潜在的TE应用。(C) 2019年Elsevier Ltd.出版
Earlier reports of fabricating 3D porous PCL scaffolds for tissue engineering applications were overshadowed by several limitations such as additional molds cost, relatively low efficiency, and lacking process control. In present study, combined extrusion-based cryogenic 3D printing (ECP) (-20 degrees C) and subsequent freeze-drying approaches were employed to facilely fabricate polycaprolactone (PCL) scaffolds, with high porosity and fidelity. Freeze-drying caused shrinkage of the scaffolds along X, Y, and Z-axes to some extent. The porosities of CP600, CP800, and CP1000 were found to be 64.0 +/- 1.2%, 70.1 +/- 1.3%, and 74.3 +/- 0.6%, respectively. The fabricated scaffolds were characterized for various structural features and compared with the ones fabricated through traditional extrusion-based melt 3D printing (EMP). The crystallinity of PCL in ECP scaffolds was lower (57.1 +/- 2.2%) than EMP scaffolds (69.8 +/- 1.3%). The ECP scaffolds showed high alkaline degradation, but low compression properties. The ECP scaffolds promoted the adhesion and proliferation of MCT3T-E1 cells with well-spread morphology on the porous filaments. Together, these features justify the suitability of printed PCL scaffolds for potential TE applications. (C) 2019 Published by Elsevier Ltd.