Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro
Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro
复制标题
丝氨酸蛋白酶抑制剂4-(2-氨基乙基)苯磺酰氟盐酸盐(AEBSF)体内抑制大鼠胚胎着床并干扰体外细胞粘附
DOI：
10.1016/j.contraception.2011.03.017
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发表时间：
2011-12-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Jiang, Ya-hong;Shi, Yan;Wang, Jian
背景：本研究旨在观察4-(2-氨基乙基)苯磺酰氟化物盐酸盐(AEBSF)对大鼠胚胎着床的体内作用及体外对细胞黏附的影响。研究设计：于妊娠第3天宫内注射AEBSF(每只角5 mg和10 mg)或尾静脉注射AEBSF(每只大鼠10 mg)，计数妊娠第8天可见着床胚胎数，以确定AEBSF的抗着床效果。分别采用小鼠胚泡-子宫内膜细胞和人脐静脉内皮细胞-HeLa细胞共培养模型检测AEBSF对细胞黏附的影响。结果：4-(2-氨基乙基)苯磺酰氟盐酸盐对大鼠胚胎着床有明显的体内抑制作用。在体外，AEBSF可干扰子宫内膜细胞上胚泡的生长，抑制HeLa细胞与人脐静脉内皮细胞的黏附。结论：4-(2-氨基乙基)苯磺酰氟盐酸盐可能是一种潜在的新型避孕药的先导化合物，其抑制着床的作用可能与干扰细胞外基质重塑过程有关。(C)2011 Elsevier Inc.保留所有权利。
Background: This study was conducted to observe the in vivo effect of 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) on embryo implantation in rats and its in vitro effect on cell adhesion.Study Design: The anti-implantation efficacy of AEBSF in rats was determined by counting the number of visible implanted embryos on day 8 of pregnancy following intrauterine (5 mg and 10 mg AEBSF per horn) or tail vein (10 mg AEBSF per rat) administration on day 3 of pregnancy. The effects of AEBSF on cell adhesion were detected, respectively, by using the mouse blastocysts-endometrial cells or the human umbilical vein endothelial cells (HUVECs)-HeLa cells co-culture model. The alteration in protein secretion pattern of HUVECs and HeLa cells was detected by the proteome analysis.Results: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride showed an in vivo inhibitory effect on embryo implantation in rat. In vitro, AEBSF could disturb the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs. The treatment of AEBSF could alter the protein secretion pattern of co-cultured HUVEC-HeLa cells.Conclusion: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride might be a potential leading compound for novel contraceptives, and its inhibitory effect on implantation might result from the interference in extracellular matrix remodeling process. (C) 2011 Elsevier Inc. All rights reserved.