Spatial‐resolved analysis of histological and biochemical alterations induced by water‐soaking in melon fruit
Spatial‐resolved analysis of histological and biochemical alterations induced by water‐soaking in melon fruit
复制标题
甜瓜果实水浸泡引起的组织学和生化变化的空间分辨分析
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
A. Graziana
中科院分区：
文献类型：
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作者：
C. Chatenet;A. Latché;E. Olmos;B. Ranty;M. Charpenteau;R. Ranjeva;J. Pech;A. Graziana
水浸泡是一种生理失调，其特征是果肉呈玻璃状，这大大降低了早期夏伦台哈密瓜（Cucumis melo L. cv.）的商业品质。旧式大披肩)。虽然人们普遍认为基因型和一些生理和环境因素在该综合征的发展中起作用，但水浸泡的内在机制尚不清楚。我们在这里报告了一项综合研究的发展在水果中浸水。利用核磁共振（NMR）成像，我们已经表明，水的流动性在患病组织增加。细胞壁的改变和大的细胞间隙的存在与细胞壁钙的严重消耗有关。浸水发生在果实成熟后期，但与乙烯生物合成没有相关性。因此，乙烯作用被1-甲基环丙烯阻断的水果对水浸泡仍然敏感。此外，两个编码乙烯生物合成关键酶的基因的表达在水浸泡过程中保持不变。观察到的主要变化涉及钙信号传导过程中的一种蛋白质。虽然钙调素总含量（普遍存在的钙结合蛋白）没有改变，但一种特殊的钙调素结合蛋白（CaM-BP）在水浸泡的组织中缺失，而在健全的成熟组织中则没有。这种CaM-BP可能是这种生理障碍的标志或决定因素。
Water-soaking, a physiological disorder characterised by a glassy texture of the flesh, depreciates greatly the commercial quality of early-season Charentais cantaloupe melons (Cucumis melo L. cv. Talma). Although it is accepted that the genotype and a number of physiological and environmental factors play a role in the development of the syndrome, the intimate mechanisms responsible for water-soaking remain unknown. We report here on an integrated study of the development of water-soaking in fruit. Using nuclear magnetic resonance (NMR) imaging, we have shown that water mobility increased in the diseased tissues. Alteration of the cell wall and the presence of large intercellular spaces were correlated with a severe depletion of cell wall calcium. Water-soaking developed during the late stages of fruit ripening, but no correlation was found with ethylene biosynthesis. Thus, fruits in which ethylene action was blocked by 1-methylcyclopropene remained sensitive to water-soaking. Moreover, the expression of two genes encoding key enzymes in ethylene biosynthesis remained unchanged in response to water-soaking. The major changes observed concerned a protein implicated in calcium signalling processes. While the amount of total calmodulin, the ubiquitous calcium binding protein, was not modified, a particular calmodulin-binding protein (CaM-BP) was absent in water-soaked but not in sound mature tissues. This CaM-BP may be a marker or a determinant of this physiological disorder.
设计用于位点特异性诱变的钙调蛋白基因的化学合成和表达。
影响因子：
2.9
作者：
Roberts,DM;Crea,R;Malecha,M;Alvarado-Urbina,G;Chiarello,RH;Watterson,DM
通讯作者：
Watterson,DM