Behavior of plastid nucleoids during male gametogenesis inPlumbago auriculata
Behavior of plastid nucleoids during male gametogenesis inPlumbago auriculata
复制标题
白花雄配子发生过程中质体核的行为
DOI：
10.1007/bf02673866
复制
发表时间：
2007
期刊：
影响因子：
2.9
通讯作者：
T. Kuroiwa
中科院分区：
文献类型：
--
作者：
C. Saito;N. Nagata;A. Sakai;H. Kuroiwa;T. Kuroiwa
我们在三维空间中研究了黑穗白花丹雄配子发生过程中质体（pt）和线粒体（mt）类核的行为。pt类核团和mt类核团在雄配子发生过程中的行为是不同的，pt类核团在小孢子早期聚集在细胞核周围，在生殖中期聚集在花粉内侧，在生殖晚期聚集在花粉外侧。我们还研究了卵和中央细胞的细胞器类核的方法，其中树脂包埋花药和子房的半厚切片，共聚焦激光扫描显微镜观察。精细胞中pt-核的数量与含DNA细胞器的行为在雄性配子发生过程中受到严格调控的结果没有显著差异。auriculata，和双亲遗传的质体在白花丹胚胎是更有利的比以前认为的。
We characterized the behavior of plastid (pt) and mitochondrial (mt) nucleoids during malegametogenesis inPlumbago auriculatain three dimensions. The behavior of pt-nucleoids and mt-nucleoids differed throughout male gametogenesis.Pt-nucleoids were distibuted in a characteristic manner in three stages: in the early microspore, pt-nucleoids assemble around cell nucleus: in the mid-generative cell,pt-nucleoids gather at the internal side of the pollen: in the late-generative cell, pt-nucleoids aggregation turns its pole to the external side of the pollen. We also studied organelle nucleoids in the egg and the central cell by a method in which semi-thick sections of resin-embedded anthers and ovaries were observed by confocal laser scanning microscopy. The number of pt-nucleoids in the sperm cell did not differ significantly from that the behavior of DNA-containing organelles is regulated strictly during male gametogenes inP. auriculata, and that a biparental inheritance of plastids in the Plumbago embryo is more favored than was previously thought.