Bioelectrical Coupling of Single-Cell States in Multicellular Systems
Bioelectrical Coupling of Single-Cell States in Multicellular Systems
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DOI：
10.1021/acs.jpclett.0c00641
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发表时间：
2020-05-07
影响因子：
5.7
通讯作者：
Mafe, Salvador
中科院分区：
文献类型：
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作者：
Cervera, Javier;Levin, Michael;Mafe, Salvador
在非兴奋细胞中，调节生化途径的信号离子和分子的时空分布受到多细胞电位的影响。这些潜力作为分布式控制器编码的发展和再生的指导性空间格局。我们回顾实验事实，并讨论最近的生物电模型，提供新的物理见解和补充生化方法。由于相邻细胞之间的耦合，单细胞状态在多细胞水平上被调制，从而允许记忆和多细胞模式。该模型是基于（i）两个通用的电压门控离子通道，促进极化和去极化的细胞状态，（ii）的转录和生物电调节的反馈机制，和（iii）电压门控细胞间电导，允许一个动态的细胞间连接。这些模拟提供了稳态和振荡的多细胞状态，有助于解释发育的各个方面，并指导实验程序，试图建立基于电势和电流的指导性生物电模式，以调节细胞行为和形态发生。
The spatiotemporal distributions of signaling ions and molecules that modulate biochemical pathways in nonexcitable cells are influenced by multicellular electric potentials. These potentials act as distributed controllers encoding instructive spatial patterns in development and regeneration. We review experimental facts and discuss recent bioelectrical models that provide new physical insights and complement biochemical approaches. Single-cell states are modulated at the multicellular level because of the coupling between neighboring cells, thus allowing memories and multicellular patterns. The model is based on (i) two generic voltage-gated ion channels that promote the polarized and depolarized cell states, (ii) a feedback mechanism for the transcriptional and bioelectrical regulations, and (iii) voltage-gated intercellular conductances that allow a dynamic intercellular connectivity. The simulations provide steady-state and oscillatory multicellular states that help explain aspects of development and guide experimental procedures attempting to establish instructive bioelectrical patterns based on electric potentials and currents to regulate cell behavior and morphogenesis.