Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry
Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry
复制标题
人精子冷冻保存的定量蛋白质组学表征：使用数据独立的采集质谱法
DOI：
10.1186/s12894-019-0565-2
复制
发表时间：
2019-12-16
期刊：
影响因子：
2
通讯作者：
Gu, Yiqun
中科院分区：
文献类型：
--
作者：
Fu, Longlong;An, Qi;Gu, Yiqun
背景人类精子冷冻保存是一种简单有效的男性生育力保存方法。方法为了识别这一过程中潜在的蛋白质组变化，采用高定量精度和高重复性蛋白质组学技术——数据独立采集（DIA）技术对人精子冷冻保存的蛋白质组学进行定量表征。结果新鲜精子和冷冻精子之间总共鉴定出 174 个显着差异的蛋白质：冷冻组中有 98 个蛋白质减少，76 个蛋白质增加。生物信息学分析表明，代谢途径在冷冻保存中发挥着重要作用，包括：丙酸代谢、乙醛酸和二羧酸代谢、糖酵解/糖异生和丙酮酸代谢。通过蛋白质印迹鉴定了四种参与糖酵解的不同蛋白质：GPI、LDHB、ADH5 和 PGAM1。结论我们的工作将为未来涉及精子冷冻保存的调查和病理学研究提供有价值的信息。
Background Human sperm cryopreservation is a simple and effective approach for male fertility preservation. Methods To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation. Results A total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1. Conclusions Our work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.