Bacterial cell shape control by nutrient-dependent synthesis of cell division inhibitors
Bacterial cell shape control by nutrient-dependent synthesis of cell division inhibitors
复制标题
DOI：
10.1016/j.bpj.2021.04.001
复制
发表时间：
2021-06-01
影响因子：
3.4
通讯作者：
Banerjee, Shiladitya
中科院分区：
文献类型：
--
作者：
Ojkic, Nikola;Banerjee, Shiladitya
通过分析枯草芽孢杆菌在营养扰动、蛋白质消耗和抗生素处理下的细胞大小和形状，我们发现细胞几何结构非常健壮，反映在表面积(S)和体积(V)之间的守恒比例关系，S类似于V-伽马，伽马=0.85.我们开发了一个由单细胞模拟支持的分子模型来预测表面到体积的比例指数伽马是由营养相关的代谢酶的产生调节的，代谢酶在细菌中充当细胞分裂抑制物。利用实验数据支持的理论，我们预测了不同细菌物种的细胞形态转换模式，并提出了细胞形态对不同营养扰动的适应机制。对于具有高表面体积比例指数伽马的生物，如枯草杆菌，细胞宽度对生长速度的变化不敏感，而具有低伽马的生物，如鲍曼不动杆菌，细胞形状容易适应生长速度的变化。
By analyzing cell size and shapes of the bacterium Bacillus subtilis under nutrient perturbations, protein depletion, and antibiotic treatments, we find that cell geometry is extremely robust, reflected in a well-conserved scaling relation between surface area (S) and volume (V), S similar to V-gamma, with gamma = 0.85. We develop a molecular model supported by single-cell simulations to predict that the surface-to-volume scaling exponent gamma is regulated by nutrient-dependent production of metabolic enzymes that act as cell division inhibitors in bacteria. Using theory that is supported by experimental data, we predict the modes of cell shape transformations in different bacterial species and propose a mechanism of cell shape adaptation to different nutrient perturbations. For organisms with high surface-to-volume scaling exponent gamma, such as B. subtilis, cell width is not sensitive to growth-rate changes, whereas organisms with low gamma, such as Acinetobacter baumannii, cell shape adapts readily to growth-rate changes.