Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard.
Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard.
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高光谱体积相干反stokes拉曼散射显微镜:定量体积测定和NaCl作为非谐波标准。
DOI:
10.1002/jrs.4876
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发表时间:
2016-09
影响因子:
2.5
通讯作者:
Langbein, Wolfgang
中科院分区:
文献类型:
--
作者:
Karuna, Arnica;Masia, Francesco;Borri, Paola;Langbein, Wolfgang
In this work, we demonstrate quantitative volume determination of chemical components in three dimensions using hyperspectral coherent anti‐Stokes Raman scattering microscopy, phase‐corrected Kramers–Kronig retrieval of the coherent anti‐Stokes Raman scattering susceptibility and factorization into concentration of chemical components. We investigate the influence of the refractive index contrast between water and polymer beads (polystyrene and polymethylmethacrylate), showing that it leads mainly to concentration errors, while the spectral error is less affected. The volume of polystyrene beads of sizes from 200 nm to 3 μm is determined with 10% relative error and 1% absolute error in the region of interest. We furthermore establish the use of sodium chloride as non‐resonant reference material free of Raman‐active vibrational resonances. © 2016 The Authors Journal of Raman Spectroscopy Published by John Wiley & Sons Ltd.
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影响因子:
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作者:
MALITSON, IH
通讯作者:
MALITSON, IH
影响因子:
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Borri, Paola
影响因子:
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通讯作者:
Langbein, Wolfgang
影响因子:
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