Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length.
Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length.
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DOI：
10.1016/j.peptides.2013.11.016
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发表时间：
2014-01
期刊：
影响因子：
3
通讯作者：
Sayegh AI
中科院分区：
文献类型：
--
作者：
Washington MC;Salyer S;Aglan AH;Sayegh AI
我们先前已经证明，腹腔内（i.p）给予胃泌素释放肽-27（GRP-27）或蛙皮素（BN）（0.21、0.41和1.03 nmol/kg）可减少进餐量（MS）并缩短餐间间隔（IMI）。在此，我们假设相同剂量的GRP-27和BN的静脉内（i.v）给药将与i.p给药在引起这些进食反应方面一样有效。为了检验这一假设，我们静脉内给予GRP-27和BN，并在整夜禁食但不缺水的雄性Sprague道利大鼠中测量第一MS（10%蔗糖）、IMI、饱腹感比率（SR，IMI/MS）和第二MS。我们发现（1）只有GRP-27降低了第一餐的MS，（2）BN延长了IMI，（3）GRP-27和BN增加了SR，（4）只有BN降低了第二餐的大小。与我们的假设相反，GRP-27和BN的i.v给药与i.p给药相比对MS和IMI的影响不同。总之，这项药理学研究表明，MS和IMI的调节在不同的网站。
We have previously shown that the intraperitoneal (i.p) administration of gastrin-releasing peptide-27 (GRP-27) or bombesin (BN) (at 0.21, 0.41 and 1.03 nmol/kg) reduces meal size (MS) and prolongs the intermeal interval (IMI). Here, we hypothesized that the intravenous (i.v) administration of the same doses of GRP-27 and BN will be as effective as the i.p administration in evoking these feeding responses. To test this hypothesis, we administered GRP-27 and BN i.v and measured first MS (10% sucrose), IMI, satiety ratio (SR, IMI/MS) and second MS in overnight food-deprived but not water-deprived male Sprague Dawley rats. We found that (1) only GRP-27 reduced the first MS, (2) BN prolonged the IMI, (3) GRP-27 and BN increased the SR and (4) only BN reduced the size of the second meal. Contrary to our hypothesis, the i.v administration of GRP-27 and BN affected the MS and IMI differently than did the i.p administration. In conclusion, this pharmacological study suggests that the MS and IMI are regulated at different sites.