Expression of the boc gene during murine embryogenesis
Expression of the boc gene during murine embryogenesis
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DOI：
10.1002/dvdy.10063
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发表时间：
2002-03-01
影响因子：
2.5
通讯作者：
Krauss, RS
中科院分区：
文献类型：
--
作者：
Mulieri, PJ;Kang, JS;Krauss, RS
BOC是一种受体样蛋白，与相关因子CDO一起，属于细胞表面分子免疫球蛋白超家族中新发现的一个亚家族。BOC和CDO形成复合体，在体外正向调节肌肉发生。为了更好地了解BOC在脊椎动物胚胎发生中的作用以及在体内是否与CDO协同作用，我们对BOC在E7.0-E17.5小鼠胚胎中的表达进行了广泛的原位杂交分析。我们的结果表明，BOC在小鼠胚胎发育过程中广泛表达，在空间和时间上都受到限制。总体而言，在E10.5和E15.5之间检测到最高水平的BOC表达，在发育中的肌肉骨骼和中枢神经系统中观察到最强的信号。在发育的后期，BOC的表达变得更加受限，主要局限于含有增殖细胞和/或未分化细胞的区域。这种表达模式与CDO的表达模式惊人地相似(Mulieri等人，2000年)。CDO和BOC在体内的重叠表达模式，再加上CDO/BOC复合体在培养细胞中促进肌肉形成，强烈表明这些蛋白在脊椎动物胚胎发生过程中共同决定、分化或两者兼而有之。(C)2002年Wiley-Liss，Inc.
BOC is a receptor-like protein that, with the related factor CDO, belongs to a newly recognized subfamily within the immunoglobulin superfamily of cell-surface molecules. BOC and CDO form complexes that positively regulate myogenesis in vitro. To gain a better understanding of the role of boc during vertebrate embryogenesis and whether it could cooperate with cdo in vivo, we carried out an extensive in situ hybridization analysis of boc expression in mouse embryos from E7.0 to E17.5. Our results show that boc is widely expressed during murine embryogenesis, in a spatially and temporally restricted pattern. Overall, the highest levels of boc expression are detected between E10.5 and E15.5, with the strongest signals observed in the developing musculoskeletal and central nervous systems. At late stages of development, boc expression becomes more restricted and is limited primarily to regions harboring proliferating cells, undifferentiated cells, or both. This expression pattern is strikingly similar to that described for cdo (Mulieri et al., 2000). The overlapping expression patterns of cdo and boc in vivo, combined with the promotion of myogenesis by CDO/BOC complexes in cultured cells, strongly suggests that these proteins play a role together in the determination, differentiation, or both, of numerous cell types during vertebrate embryogenesis. (C) 2002 Wiley-Liss, Inc.