Cu2+-mediated complex formation between polyacrylic acid (PAA) and bovine serum albumin
Cu2+-mediated complex formation between polyacrylic acid (PAA) and bovine serum albumin
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DOI：
10.1016/0022-1759(96)00107-x
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发表时间：
1996-10-16
影响因子：
2.2
通讯作者：
Bermek, E
中科院分区：
文献类型：
--
作者：
Mustafaev, MI;Yucel, F;Bermek, E
在中性水中研究了Cu2+介导的聚丙烯酸（PAA）与带负电荷的牛血清白蛋白（BSA）之间的络合物形成。根据Cu2+的浓度，PAA-Cu2+配合物与BSA之间的反应似乎遵循两种可能的途径之一。在低Cu2+浓度(n（Cu)/n(AA) < 0.15）下，BSA浓度的进一步增加导致配合物的分解，机制一：[图形]在高Cu2+浓度(n（Cu)/n(AA) > 0.15）下，BSA浓度的进一步增加导致非化学计量多配合物的形成（机制二）：[图形]研究了BSA-Cu2+-PAA三元混合物的免疫原性，并分析了溶液中免疫原性与配合物形成的关系。在含有BSA的PAA溶液中加入Cu2+可显著提高BSA特异性免疫原性。对不同配比组成的BSA-Cu2+-PAA复合物的免疫原性分析表明：(1)稳定的三元配合物具有最高的免疫原性活性，(2)具有免疫活性的多电解质复合物具有非化学计量组成。因此，我们提出了一种基于Cu2+介导的复合物形成的新方法，以增强蛋白质特异性抗体反应。
Cu2+-mediated complex formation between polyacrylic acid (PAA) and negatively charged bovine serum albumin (BSA) was studied in neutral water in the presence of Cu2+. Depending on the concentration of Cu2+, the reaction between PAA-Cu2+ complexes and BSA appeared to follow one of two possible paths. At low Cu2+ concentrations (n(Cu)/n(AA) < 0.15), a further increase in BSA concentration led to the breakdown of the complex as in mechanism I:[GRAPHICS]At higher Cu2+ concentrations (n(Cu)/n(AA) > 0.15), a further increase in BSA concentration led to the formation of non-stoichiometric polycomplexes (mechanism II):[GRAPHICS]The immunogenic properties of ternary mixtures of BSA-Cu2+-PAA were investigated and the relationship between immunogenicity and complex formation in solution was analyzed. The addition of Cu2+ to solutions of PAA with BSA gave rise to a considerable increase in BSA-specific immunogenicity. Data obtained from the analysis of the immunogenicity of BSA-Cu2+-PAA mixtures formed using different ratios of the components suggested that (1) the highest immunogenic activity is exhibited by stable ternary complexes, and (2) immunoactive polyelectrolyte complexes have a non-stoichiometric composition. We thus propose a novel method, based on Cu2+ mediated complex formation, to enhance protein-specific antibody responses.