Identification of New Peptide Biomarkers for Bacterial Bloodstream Infection
Identification of New Peptide Biomarkers for Bacterial Bloodstream Infection
复制标题
细菌血流感染的新肽生物标志物的鉴定
DOI：
10.1002/prca.201900075
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发表时间：
2019-12-10
影响因子：
2
通讯作者：
Wang, Chengbin
中科院分区：
文献类型：
--
作者：
Ma, Yating;Wen, Xinyu;Wang, Chengbin
目的由于缺乏有效的早期诊断措施，迫切需要新的细菌性血流感染(BSI)诊断方法。蛋白质-多肽图谱方法可用于识别BSI的新的诊断生物标记物。实验设计本研究采用MALDI-TOF MS和Nano-LC/ESI-MS/MS分析血清中的多肽。此外，还进行了GO和网络分析，作为分析这些潜在蛋白质标记的一种手段。最后，通过酶联免疫吸附试验在独立的临床样本中验证潜在的生物标志物。结果m/z 1533.8、2794.3、3597.3、5007.3、7816.7的变化趋势一致，感染组m/z 1533.8、2794.3、3597.3的强度高于对照组，而m/z 5007.3、7816.7的强度低于对照组。共鉴定出4个峰，分别为ITIH4、KNG1、SAA2和C3。GO和网络分析发现这些蛋白质形成了一个相互作用的网络，这可能与BSI相关。酶联免疫吸附试验结果表明，ITIH4、KNG1和SAA2能有效地区分感染与正常对照组和发热组。结论和临床相关性：这些生物标记物有可能为BSI的发生和发展提供新的信号网络。
Purpose Due to a lack of effective early diagnostic measures, new diagnostic methods for bacterial bloodstream infections (BSIs) are urgently needed. A protein-peptide profiling approach can be used to identify novel diagnostic biomarkers of BSIs. Experimental design In this study, MALDI-TOF MS and nano-LC/ESI-MS/MS are used to analyze serum peptides. In addition, GO and network analyses are conducted as a means of analyzing these potential protein markers. Finally, the potential biomarkers are verified in independent clinical samples via ELISA. Results m/z 1533.8, 2794.3, 3597.3, 5007.3, and 7816.7 reveal an identical trend; the intensity of m/z 1533.8, 2794.3, and 3597.3 are higher in the infection group relative to controls, whereas the intensity of m/z 5007.3 and 7816.7 are lower in the infection group. Four peaks are successfully identified including ITIH4, KNG1, SAA2, and C3. GO and network analyses find these proteins to form an interaction network, which may be correlated with BSI. ELISA results indicate that ITIH4, KNG1, and SAA2 are effective in differentiating infected from normal control group and the febrile group. Conclusions and clinical relevance These biomarkers have the potential to offer new insights into the signaling networks underlying the development and progression of BSI.