A Clostridium perfringens vector for the selection of promoters.
A Clostridium perfringens vector for the selection of promoters.
复制标题
用于选择启动子的产气荚膜梭菌载体。
DOI：
10.1006/plas.1994.1035
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发表时间：
1994
期刊：
影响因子：
2.6
通讯作者：
A. Okabe
中科院分区：
文献类型：
--
作者：
Chieko Matsushita;O. Matsushita;M. Koyama;A. Okabe
产气荚膜梭菌基因的启动子选择载体是从产气荚膜梭菌-大肠杆菌穿梭载体pJIR418构建的。该质粒携带无启动子氯霉素乙酰转移酶基因 (catP)，该基因源自 pIP401，位于 pUC18 多克隆位点的下游。当磷脂酶C基因的启动子区插入克隆位点之一时，携带所得质粒的产气荚膜梭菌菌株13的衍生物获得了对氯霉素的抗性。该质粒应该是产气荚膜梭菌基因有用的报告系统。
A promoter selection vector for Clostridium perfringens genes was constructed from a C. perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be useful reporter system for C. perfringens genes.