Direct laboratory evidence that pregnancy-induced hypertension might be associated with increased catecholamines and decreased renalase concentrations in the umbilical cord and mother's blood
Direct laboratory evidence that pregnancy-induced hypertension might be associated with increased catecholamines and decreased renalase concentrations in the umbilical cord and mother's blood
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DOI：
10.1515/labmed-2018-0185
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发表时间：
2019-04-01
影响因子：
1.2
通讯作者：
Aydin, Suleyman
中科院分区：
文献类型：
--
作者：
Baykus, Yakup;Ustebay, Sefer;Aydin, Suleyman
背景：肾氨酸酶(Renalase，RNL)是否氧化儿茶酚胺(Cas)是一种有争议的酶，一般认为它能氧化儿茶酚胺(CAS)。CAS(多巴胺[DPMN]、肾上腺素[EPI]和去甲肾上腺素[NEPI])与高血压有关，包括妊娠高血压，后者在所有妊娠中发生的比例为8%-10%。因此，本研究的目的是比较正常血压对照组(C)、子痫前期(PE)患者和重度子痫前期(SPE)患者的CAS和肾酶浓度。共采集血样270份，其中母亲血样90份，脐动脉血样90份，脐静脉血样90份。结果：正常孕妇与PE和SPE孕妇(C组SBP/DBP为120/80 mm Hg，PE组>140/90 mm Hg，SPE组>160/110 mm Hg)的CA、RNL和RNL含量比较，差异有统计学意义(P<0.05)。妊娠期PE和SPE孕妇的SBP/DBP与RNL含量呈负相关。结论：本研究结果表明，妊娠期PE和SPE患者的高血压与CA水平升高和RNL浓度降低有直接关系。未来可能更倾向于使用RNL制剂，以预防妊娠高血压综合征引起的孕产妇和围产儿发病率和死亡率。
Background: Renalase (RNL) is a controversial enzyme as to whether it oxidizes catecholamines (CAs) (as is generally accepted) in the blood or not. CAs (dopamine [DPMN], epinephrine [EPI] and norepinephrine [NEPI]) are associated with hypertension, including pregnancy-induced hypertension, which occurs in 8-10% of all pregnancies. Therefore, the aim of the study was to compare CAs and renalase concentration in (i) normotensive controls (C), (ii) patients with preeclampsia (PE) and (iii) patients with severe preeclampsia (SPE), which is one of the well-known symptoms of hypertension.Methods: This case-control study involved 90 women divided into three groups - 30 C, 30 PE and 30 SPE - whose age and body mass indexes (BMIs) were similar. A total of 270 blood samples (90 maternal samples, 90 umbilical cord artery samples and 90 umbilical cord vein samples) were obtained. CAs and RNL concentrations of the biological samples were measured by enzyme-linked immunosorbent assay (ELISA).Results: Comparing the amounts of CAs, RNL and systolic blood pressure (SBP)/diastolic blood pressure (DBP) between healthy control pregnant women and pregnant women with PE and SPE (SBP/DBP was 120/80 mm Hg for C, above 140/90 mm Hg for PE and above 160/110 mm Hg for SPE), the levels of CAs were significantly increased whereas RNL was reduced. The correlation between SBP/DBP and the amount of RNL in pregnant women with PE and SPE was negative.Conclusions: These novel results are evidence that hypertension seen in PE and SPE is directly related to increased levels of CAs and reduced RNL concentrations. The use of RNL preparations may be preferred in future to prevent maternal and perinatal morbidity and mortality due to pregnancy-induced hypertension.