Rapid Heteronuclear Single Quantum Correlation NMR Spectra at Natural Abundance
Rapid Heteronuclear Single Quantum Correlation NMR Spectra at Natural Abundance
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DOI：
10.1021/ja411588d
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发表时间：
2014-01-29
影响因子：
15
通讯作者：
Luy, Burkhard
中科院分区：
文献类型：
--
作者：
Schulze-Suenninghausen, David;Becker, Johanna;Luy, Burkhard
一种新的NMR实验，所谓的ASAP-HSQC，介绍了允许在小于30秒的小分子在自然丰度不妥协的扫描宽度，分辨率或光谱质量的检测杂环酮键的相关性。同样，与传统的HSQC相比，该实验允许在相同的总实验时间内显著增加数字分辨率或适度的灵敏度增强。的增益是一个结果，保持所有未使用的质子磁化沿着z在采集过程中，使以前报道的ASAP和ALSOFAST的方法可以从HMQC转移到HSQC型实验。接下来的基本和宽带脉冲序列，相对于最小的测量时间，灵敏度增益，并在分辨率的优势相比，国家的最先进的实验序列的表征。
A novel NMR experiment, the so-called ASAP-HSQC, is introduced that allows the detection of heteronuclear one-bond correlations in less than 30 s on small molecules at natural abundance without compromises in sweep width, resolution or spectral quality. Equally, the experiment allows a significant increase in digital resolution or a moderate senstitivity enhancement in the same overall experiment time compared to a conventional HSQC. The gain is a consequence of keeping all unused proton magnetization along z during acquisition, so that the previously reported ASAP and ALSOFAST approaches can be transferred from HMQC to HSQC-type experiments. Next to basic and broadband pulse sequences, a characterization of the sequence with respect to minimum measurement time, sensitivity gain, and advantages in resolution compared to state-of-the-art experiments is given.