Relative distribution of estrogen and progesterone receptors among the epithelial, adipose, and connective tissue components of the normal mammary gland.
Relative distribution of estrogen and progesterone receptors among the epithelial, adipose, and connective tissue components of the normal mammary gland.
复制标题
雌激素和孕激素受体在正常乳腺的上皮、脂肪和结缔组织成分中的相对分布。
作者：
S. Haslam;G. Shyamala
本研究旨在确定雌激素和孕激素受体（PgR）在正常小鼠乳腺上皮、脂肪和结缔组织中的相对分布，并确定PgR浓度和乳腺对PgR雌激素刺激敏感性的调节发生在哪些组织中。根据清除脂肪垫技术，在体内手术分离乳腺上皮与脂肪和结缔组织。使用[3H]R5020或[3H]雌二醇对完整乳腺或无上皮且仅含乳腺脂肪和结缔组织的透明脂肪垫的细胞质提取物进行结合研究。我们发现，乳腺脂肪和结缔组织含有单独的，高亲和力，类固醇特异性，大分子结合位点的雌激素和孕激素，和大多数的PgR只存在于腺上皮。此外，PgR浓度相对于乳腺发育的调制以及PgR浓度的雌激素调节似乎仅限于乳腺的上皮成分。由于在妊娠和哺乳期间发生的主要乳腺发育变化也发生在上皮中，因此提出受体调节可能在乳腺中起调节作用。
The present studies were undertaken to determine the relative distribution of estrogen and progesterone receptors (PgR) in the epithelial, adipose, and connective tissues of normal mouse mammary gland and to determine in which tissue(s) the modulations in PgR concentration and mammary gland sensitivity to estrogenic stimulation of PgR occur. Mammary epithelium was separated from adipose and connective tissues surgically in vivo according to the cleared fat pad technique. Binding studies using [3H]R5020 or [3H]estradiol were carried out on cytoplasmic extracts of intact mammary gland or cleared fat pads which were devoid of epithelium and contained only mammary adipose and connective tissues. We found that mammary adipose and connective tissues contain separate, high affinity, steroid-specific, macromolecular binding sites for estrogen and progesterone, and the majority of PgR are present only in the glandular epithelium. Furthermore, the modulation in PgR concentration relative to mammary gland development as well as the estrogenic regulation of PgR concentration appear to be restricted to the epithelial component of mammary gland. Since the major mammary gland developmental changes that occur during pregnancy and lactation also occur in the epithelium, it is proposed that the receptor modulations may serve a regulatory function in mammary gland.