Bacteriophages in clinical samples can interfere with microbiological diagnostic tools
Bacteriophages in clinical samples can interfere with microbiological diagnostic tools
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DOI：
10.1038/srep33000
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发表时间：
2016-09-09
影响因子：
4.6
通讯作者：
Navarro, Ferran
中科院分区：
文献类型：
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作者：
Brown-Jaque, Maryury;Muniesa, Maite;Navarro, Ferran
噬菌体是感染细菌的病毒，它们存在于细菌宿主存在的任何地方，包括人体。为了探索临床样本中是否存在腹水，我们评估了65份临床样本（血液、腹水、尿液、脑脊液和血清）。在>45%的腹水和尿液样品中检测到感染性尾丝虫。观察到噬菌体干扰细菌分离的三个实例。当接种物取自含有裂解菌斑的琼脂平板时，噬菌体可防止菌落图测定所需的融合细菌生长，但当接种物取自无噬菌体区域中的单个菌落时，则不会。此外，细菌直接从腹水中分离，但不是在相同样品的液体富集培养后分离，因为噬菌体增殖裂解了细菌。最后，在尿样中观察到的革兰氏阴性杆菌在琼脂平板上不生长，因为样品中的感染性大肠杆菌密度高。
Bacteriophages are viruses that infect bacteria, and they are found everywhere their bacterial hosts are present, including the human body. To explore the presence of phages in clinical samples, we assessed 65 clinical samples (blood, ascitic fluid, urine, cerebrospinal fluid, and serum). Infectious tailed phages were detected in >45% of ascitic fluid and urine samples. Three examples of phage interference with bacterial isolation were observed. Phages prevented the confluent bacterial growth required for an antibiogram assay when the inoculum was taken from an agar plate containing lysis plaques, but not when taken from a single colony in a phage-free area. In addition, bacteria were isolated directly from ascitic fluid, but not after liquid enrichment culture of the same samples, since phage propagation lysed the bacteria. Lastly, Gram-negative bacilli observed in a urine sample did not grow on agar plates due to the high densities of infectious phages in the sample.