Genetic diversity of HIV type 1 in rural eastern Cameroon
Genetic diversity of HIV type 1 in rural eastern Cameroon
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DOI：
10.1097/00126334-200412150-00019
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发表时间：
2004-12-15
影响因子：
3.6
通讯作者：
Ichimura, H
中科院分区：
文献类型：
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作者：
Ndembi, N;Takehisa, J;Ichimura, H
为了监测在喀麦隆东部流行的基因型HIV-1变异体的存在，从与中非共和国（CAR）接壤的农村村庄的3个当地卫生中心收集了57名HIV-1感染者的血液样本，并进行了遗传学分析。在gag和env-C2 V3聚合酶链反应（PCR）图谱均为阳性的40株HIV-1毒株中，12株（30.0%）亚型或CRF名称不一致：2例亚型B/A（gag/env）、1例B/CRF 01、2例B/CRF 02、1例CRF01/CRF01.A、2例CRF 11/CRF 01、1例CRF 13/A、1例CRF 13/CRF 01、1例CRF 13/CRF 11和1例G/U（未分类）。28个菌株（70.0%）在gag和env之间具有一致的亚型或CRF名称：27个亚型A和1个F2。在使用env-C2 V3引物的PCR阴性的其余17株HIV-1毒株中，10株（58.8%）具有不一致的亚型或CRF，7株（41.2%）具有基于gag/pol/env-gp 41分析的一致亚型。总的来说，很高比例（22/57，38.6%）的分离株被发现是重组菌株。此外，还发现了新形式的HIV-1毒株，如亚型B/A（gag/env）、B/CRF 01和B/CRF 02。在喀麦隆东部，CRF 02和CRF 11的流行率分别相对较低和较高，与中非共和国和乍得的流行率相比，CRF 02是喀麦隆其他地区最主要的HIV-1毒株，其流行病学模式与中非共和国和乍得的流行率更密切相关。这些研究结果强烈表明，喀麦隆的这一部分是HIV-1重组的潜在热点，有可能活跃产生新形式的HIV-1变体，尽管新形式的HIV-1的流行病学意义尚不清楚。
To monitor the presence of genotypic HIV-1 variants circulating in eastern Cameroon, blood samples from 57 HIV-1-infected individuals attending 3 local health centers in the bordering rural villages with Central African Republic (CAR) were collected and analyzed phylogenetically. Out of the 40 HIV-1 strains with positive polymerase chain reaction (PCR) profile for both gag and env-C2V3,12 (30.0%) had discordant subtype or CRF designation: 2 subtype B/A (gag/env), 1 B/CRF01, 2 B/CRF02, 1 CRF01/CRF01.A, 2 CRF11/CRF01, 1 CRF13/A, 1 CRF13/CRF01, 1 CRF13/CRF11, and 1 G/U (unclassified). Twenty-eight strains (70.0%) had concordant subtypes or CRF designation between gag and env: 27 subtype A and 1 F2. Out of the remaining 17 HIV-1 strains negative for PCR with the env-C2V3 primers used, 10 (58.8%) had discordant subtype or CRF, and 7 (41.2%) had concordant one based on gag/pol/env-gp41 analysis. Altogether, a high proportion (22/57, 38.6%) of the isolates were found to be recombinant strains. In addition, an emergence of new forms of HIV-1 strains, such as subtype B/A (gag/env), B/CRF01 and B/CRF02, was identified. The epidemiologic pattern of HIV-1 in eastern Cameroon, relatively low and high prevalence of CRF02 and CRF 11, respectively, was more closely related to those of CAR and Chad than that of other regions of Cameroon, where CRF02 is the most predominant HIV-1 strain. These findings strongly suggest that this part of Cameroon is a potential hotspot of HIV-1 recombination, with a likelihood of an active generation of new forms of HIV-1 variants, though epidemiologic significance of new HIV-1 forms is unknown.