CHARACTERIZATION OF 2 DROSOPHILA-MELANOGASTER CYTOCHROME-C GENES AND THEIR TRANSCRIPTS
CHARACTERIZATION OF 2 DROSOPHILA-MELANOGASTER CYTOCHROME-C GENES AND THEIR TRANSCRIPTS
复制标题
DOI：
10.1093/nar/13.2.631
复制
发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
WU, R
中科院分区：
文献类型：
--
作者：
LIMBACH, KJ;WU, R
用基因组印迹杂交技术对果蝇黑腹DNA进行分析，结果表明果蝇基因组中存在两个类细胞色素c序列。这两个序列，DC 3和DC 4，已经从Charon 4A-D.melanogastergenomic文库中分离。DC 3和DC 4位于2号染色体左臂上的位置36 A 10-11处的4kb DNA区域内。这两个克隆的核苷酸序列已经确定。DC 3和DC 4都可以编码功能性细胞色素c蛋白。然而，由这两个基因预测的多肽序列在32个氨基酸残基处不同。DC 4在整个果蝇发育过程中以不同但相对较高的水平表达。相反，DC 3在整个发育过程中以恒定但相对较低的水平表达。
Analysis of totalDrosophilamelanogasterDNA by genomic blot hybridization indicates that two cytcchrome c-like sequences exist in the Drosophila genome. These two sequences, DC3 and DC4, have been isolated from a Charon 4A–D.melanogastergenomic library. DC3 and DC4 are located within a 4 kb region of DNA, at position 36A 10–11, on the left arm of chromosome 2. The nucleotide sequence of these two clones has been determined. Both DC3 and DC4 can encode functional cytochrome c proteins. The polypeptide sequences predicted by these two genes, however, differ at 32 amino acid residues. DC4 is expressed at varying, but relatively high levels throughout Drosophila development. In contrast, DC3 is expressed at constant, but relatively low levels throughout development.