Potential Prognostic and Diagnostic Values of CDC6, CDC45, ORC6 and SNHG7 in Colorectal Cancer
Potential Prognostic and Diagnostic Values of CDC6, CDC45, ORC6 and SNHG7 in Colorectal Cancer
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CDC6、CDC45、ORC6 和 SNHG7 在结直肠癌中的潜在预后和诊断价值
DOI：
10.2147/ott.s231941
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Wang, Guo
中科院分区：
文献类型：
--
作者：
Hu, Yang;Wang, Liping;Wang, Guo
背景：结直肠癌（Colorectal cancer，CRC）是人类常见的恶性肿瘤.方法：采用Bioconductor R的affy和Limma软件包，分别对大肠癌基因数据集（GSE 8671，GSE 21510，GSE 32323，GSE 39582和TCGA）进行差异表达基因（DEG）和差异表达lncRNA（DELs）分析。应用GO和KEGG通路分析DEG基因，Kaplan-Meier生存曲线和考克斯回归分析DEG基因表达异常与大肠癌患者生存结局的关系。结果：共检测到306个表达上调的DEG和213个表达下调的DEG，共检测到485个DELs，其中241个表达上调，244个表达下调。GO和KEGG途径分析表明DEGs参与细胞周期、矿物质吸收、DNA复制和氮代谢。其中，Kaplan-Meier生存曲线和考克斯回归分析显示，CDC 6、CDC 45、ORC 6和SNHG 7水平与结直肠癌患者的生存结局显著相关。结论：CDC 6、CDC 45、ORC 6和SNHG 7在结直肠癌的发生、发展过程中起重要作用，可作为结直肠癌诊断、治疗和预后的潜在靶点。
Background: Colorectal cancer (CRC) is a common human malignancy. The aims of this study are to investigate the gene expression profile of CRC and to explore potential strategy for CRC diagnosis, therapy and prognosis.Methods: We use affy and Limma package of Bioconductor R to do differential expression genes (DEGs) and differential expression lncRNAs (DELs) analysis from the gene datasets (GSE8671, GSE21510, GSE32323, GSE39582 and TCGA) respectively. Then, DEGs were analyzed by GO and KEGG pathway and Kaplan-Meier survival curve and Cox regression analyses were used to find aberrantly expressed genes associated with survival outcome of CRC patients. Real-time PCR assay was used to verify the aberrantly expressed genes expression in CRC samples.Results: 306 up-regulation and 213 down-regulation common DEGs were found. A total of 485 DELs were identified, of which 241 up-regulated and 244 down-regulated. Then, GO and KEGG pathway analyses showed that DEGs were involved in cell cycle, mineral absorption, DNA replication, and Nitrogen metabolism. Among them, Kaplan-Meier survival curve and Cox regression analyses revealed that CDC6, CDC45, ORC6 and SNHG7 levels were significantly associated with survival outcome of CRC patients. Finally, real-time PCR assay was used to verify that the CDC6, CDC45, ORC6 and SNHG7 expression were upregulated in 198 CRC samples compared with the expression levels in individual-matched adjacent mucosa samples.Conclusion: CDC6, CDC45, ORC6 and SNHG7 are implicated in CRC initiation and progression and could be explored as potential diagnosis, therapy and prognosis targets for CRC.