Phase-Shifting the Fruit Fly Clock without Cryptochrome
Phase-Shifting the Fruit Fly Clock without Cryptochrome
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DOI：
10.1177/0748730411434390
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Helfrich-Foerster, Charlotte
中科院分区：
文献类型：
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作者：
Kistenpfennig, Christa;Hirsh, Jay;Helfrich-Foerster, Charlotte
蓝光感光色素隐花色素（CRY）被认为是果蝇的主要昼夜感光感受器。然而，在没有功能性 CRY 的情况下，明暗循环的夹带也是可能的。在这里，我们监测了cry(01)突变体和对照果蝇对1小时1000勒克斯光脉冲的相位响应曲线。我们发现cry(01)突变体在主观清晨和傍晚时分改变其活动节律，尽管幅度减小。这种相移能力足以减缓突变体的夹带速度，表明眼睛有助于时钟在黎明和黄昏时的光敏感性。对于较长的光脉冲（3小时和6小时），野生型果蝇表现出显着增强的相移幅度，但无CRY的果蝇似乎以野生型方式整合光脉冲持续时间的能力受损：只有昼夜节律时间21的6小时光脉冲显着增加了cry（01）突变体的相移幅度。在昼夜节律时间 15，突变体表现出相位提前而不是预期的延迟。对这些复杂的结果进行了讨论。
The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry(01) mutants and control flies to 1-hour 1000-lux light pulses. We found that cry(01) mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry(01) mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.