Widespread occurrence of antisense transcription in the human genome
Widespread occurrence of antisense transcription in the human genome
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DOI：
10.1038/nbt808
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发表时间：
2003-04-01
影响因子：
46.9
通讯作者：
Rotman, G
中科院分区：
文献类型：
--
作者：
Yelin, R;Dahary, D;Rotman, G
越来越多的真核基因被发现具有天然存在的反义转录物。在这里，我们研究了人类基因组中的反义转录的程度，通过分析公共数据库中的表达序列，使用一组计算工具，旨在确定正义反义转录单位对相反的DNA链相同的基因组位点。通过含有来自重叠区域的链特异性寡核苷酸探针的微阵列评估2，667个有义-反义对的所得数据集。通过用链特异性核糖核酸探针进行的北方印迹分析对特定情况的验证证明了来自两条DNA链的转录。我们的结论是，大于或等于60%的数据集，或约1,600个预测的正义-反义转录单位，从两条DNA链转录。这表明通常被认为是罕见的反义转录的发生在人类基因组中是一种非常普遍的现象。因此，人类细胞中基因表达的反义调节可能是一种常见的调节机制。
An increasing number of eukaryotic genes are being found to have naturally occurring antisense transcripts. Here we study the extent of antisense transcription in the human genome by analyzing the public databases of expressed sequences using a set of computational tools designed to identify sense-antisense transcriptional units on opposite DNA strands of the same genomic locus. The resulting data set of 2,667 sense-antisense pairs was evaluated by microarrays containing strand-specific oligonucleotide probes derived from the region of overlap. Verification of specific cases by northern blot analysis with strand-specific riboprobes proved transcription from both DNA strands. We conclude that greater than or equal to60% of this data set, or -1,600 predicted sense-antisense transcriptional units, are transcribed from both DNA strands. This indicates that the occurrence of antisense transcription, usually regarded as infrequent, is a very common phenomenon in the human genome. Therefore, antisense modulation of gene expression in human cells may be a common regulatory mechanism.