Production and characterization of poly(3-hydroxybutyrateco-3-hydroxyhexanoate)-poly(ethylene glycol) hybird copolymer with adjustable molecular weight
Production and characterization of poly(3-hydroxybutyrateco-3-hydroxyhexanoate)-poly(ethylene glycol) hybird copolymer with adjustable molecular weight
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分子量可调的聚(3-羟基丁酸酯-3-羟基己酸酯)-聚乙二醇杂化共聚物的制备及表征
DOI：
10.1007/s10118-012-1095-5
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发表时间：
2012-01-01
影响因子：
4.3
通讯作者：
Ma, Jian-gang
中科院分区：
文献类型：
--
作者：
Zhang, Ya-li;Lu, Xiao-yun;Ma, Jian-gang
以聚乙二醇（PEG，Mn= 200）为原料，利用气单胞菌4AK 4发酵合成了一种新型天然-合成杂化嵌段共聚物。该杂化生物材料由亲水性PEG封端的天然疏水性聚合物聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）组成，其具有增加的柔性以及改善的热稳定性。二甘醇（DEG）和乙二醇的加入不会导致杂化嵌段共聚物的积累。DEG和乙二醇与PEG-200共同作用可导致PHBHHx的摩尔质量降低，生成一系列低分子量聚合物，聚合物产率和细胞生产率降低，不同分子量的PHBHHx和PHBHHx-PEG的体外降解表明，分子量的降低加速了共聚物的降解，PEG改性对其降解率影响不大。本研究结果为制备一系列分子量可调、分子量分布较宽的PHBHHx和PHBHHx-PEG材料提供了一种简便、直接的方法，具有重要的生物医学应用价值。
A novel natural-synthetic hybrid block copolymer was synthesized byAeromonas hydrophila4AK4 in poly(ethylene glycol) (PEG,Mn= 200) modified fermentation. This hybrid biomaterial consists of the natural hydrophobic polymer poly(3-hydroxybutyrat-co-3-hydroxyhexanoate) (PHBHHx) end-capped with hydrophilic PEG, which has the increased flexibility as well as the improved thermal stability. Addition of diethylene glycol (DEG) and ethylene glycol could not result in the accumulation of hybrid block copolymer. DEG and ethylene glycol, together with PEG-200, could cause a reduction of molar mass of PHBHHx, resulting in a series of low molecular weight polymer and the reduction of the polymer yield as well as the cellular productivity.In vitrodegradation of PHBHHxand PHBHHx-PEG with different molecular weight showed that the decrease of molecular weight accelerated the degradation of copolymers, but PEG modification has little effect on its degradation rate. The results in this study provided a convenient and direct method to produce a series of PHBHHx and PHBHHx-PEG materials with adjustable molecular weight and broad molecular weight distribution which will be very useful for the biomedical applications.