Nop5 interacts with the archaeal RNA exosome
Nop5 interacts with the archaeal RNA exosome
复制标题
DOI：
10.1002/1873-3468.12915
复制
发表时间：
2017-12-01
期刊：
影响因子：
3.5
通讯作者：
Klug, Gabriele
中科院分区：
文献类型：
--
作者：
Gauernack, A. Susann;Lassek, Christian;Klug, Gabriele
古细菌外泌体是一种负责 RNA 磷酸解降解和拖尾的蛋白质复合物，具有包含 Rrp4、Csl4 和 DnaG 的 RNA 结合平台。为了检测外泌体的新相互作用伙伴，我们共纯化了 Nop5，它是 rRNA 甲基化核糖核蛋白复合物的一部分，与生长到后期稳定期的硫磺菌外泌体一起纯化。我们证明了 Nop5 能够通过同源三聚体 Rrp4-cap 与外泌体结合，并在体外增加多腺苷酸化 RNA 的比例，这表明 Nop5 是一种双功能蛋白。由于RNA的拖尾可能有助于增强RNA降解，因此Nop5与稳定期古细菌外泌体的结合可能会增强RNA的拖尾和降解，作为生存策略。
The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the exosome of Sulfolobus solfataricus grown to a late stationary phase. We demonstrated the capability of Nop5 to bind to the exosome with a homotrimeric Rrp4-cap and to increase the proportion of polyadenylated RNAin vitro, suggesting that Nop5 is a dual-function protein. Since tailing of RNA probably serves to enhance RNA degradation, association of Nop5 with the archaeal exosome in the stationary phase may enhance tailing and degradation of RNA as survival strategy.