SAS-4 is a C-elegans centriolar protein that controls centrosome size
SAS-4 is a C-elegans centriolar protein that controls centrosome size
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DOI：
10.1016/s0092-8674(03)00117-x
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发表时间：
2003-02-21
期刊：
影响因子：
64.5
通讯作者：
Hyman, AA
中科院分区：
文献类型：
--
作者：
Kirkham, M;Müller-Reichert, T;Hyman, AA
中心体由一对中心粒组成，周围环绕着中心粒周围的物质(PCM)。以往的工作表明，需要中心粒来组织PCM形成结构稳定的细胞器。在这里，我们描述了秀丽隐杆线虫中的中心粒成分SAS-4。与微管蛋白一样，SAS-4在复制过程中被合并到中心粒中，并在复制后保持稳定的联系。在没有SAS-4的情况下，中心粒复制减少。SAS-4的部分耗尽导致结构缺陷的中心粒，这些中心粒含有降低的SAS-4水平，并组织成比例较少的PCM。因此，SAS-4是一个中心粒相关的成分，其数量决定了中心体的大小。这些结果提供了对中心粒作为中心体组织者的鲜为人知的作用的新见解。
Centrosomes consist of a centriole pair surrounded by pericentriolar material (PCM). Previous work suggested that centrioles are required to organize PCM to form a structurally stable organelle. Here, we characterize SAS-4, a centriole component in Caenorhabditis elegans. Like tubulin, SAS-4 is incorporated into centrioles during their duplication and remains stably associated thereafter. In the absence of SAS-4, centriole duplication falls. Partial depletion of SAS-4 results in structurally defective centrioles that contain reduced levels of SAS-4 and organize proportionally less PCM. Thus, SAS-4 is a centriole-associated component whose amount dictates centrosome size. These results provide novel insight into the poorly understood role of centrioles as centrosomal organizers.