Cell-derived micro-environment helps dental pulp stem cells promote dental pulp regeneration
Cell-derived micro-environment helps dental pulp stem cells promote dental pulp regeneration
复制标题
细胞源性微环境帮助牙髓干细胞促进牙髓再生
DOI：
10.1111/cpr.12361
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发表时间：
2017-10-01
影响因子：
8.5
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Zhang, Xuexin;Li, Hui;Tian, Weidong
目的牙髓的功能与细胞外基质（extracellular matrix， ECM）结构密切相关，ECM因其调节细胞的生物学功能而备受关注。因此，ECM生态位和细胞之间的相互作用值得探索潜在的临床应用。材料和方法本研究通过细胞培养和脱细胞制备牙髓干细胞（DPSC）来源的ECM （DPM）作为细胞生态位，并评价DPM培养后DPSC行为的变化和牙髓组织再生的组织学分析。DPM促进了DPSCs的复制，并保留了它们的矿化。然后，我们进一步研究了基于dpm的成牙培养基培养策略，矿化相关标记物显示DPSCs受到成牙分化的调控。DPM应用于裸鼠皮下植入2个月后，获得排列整齐的牙髓样组织。此外，在塑料培养表面培养的DPSCs在体外矿化生成因子上调，但在体内培养时，细胞的基质形成和矿化出现紊乱。结果与结论基于sdpm的培养可以作为细胞生态位，调节DPSC的行为，为组织特异性ECM的获取提供了一种替代方法，并为ECM-细胞相互作用提供了策略。
ObjectivesThe function of the dental pulp is closely connected to the extracellular matrix (ECM) structure, and ECM has received significant attention due to its biological functions for regulating cells. As such, the interaction between the ECM niche and cells is worth exploring for potential clinical uses.Materials and methodsIn this study, dental pulp stem cell (DPSC)-derived ECM (DPM) was prepared through cell culture and decellularization to function as the cell niche, and changes in DPSC behaviour and histological analysis of dental pulp tissue regeneration were evaluated following the DPM culture. DPM promoted the replication of DPSCs and exhibited retention of their mineralization. Then, the DPM-based culture strategy under odontogenic culture medium was further investigated, and the mineralization-related markers showed that DPSCs were regulated towards odontogenic differentiation. Dental pulp-like tissue with well-arranged ECM was harvested after a 2-month subcutaneous implantation in nude mice with DPM application. Additionally, DPSCs cultured on the plastic culture surface showed the up-regulation of mineralization makers in vitro, but there was a disorder in matrix formation and mineralization when the cells were cultured in vivo.Results and ConclusionsDPM-based cultivation could serve as a cell niche and modulate DPSC behaviour, and this method also provided an alternative to harvest tissue-specific ECM and provided a strategy for ECM-cell interaction.