Oral Administration of L-Citrulline, but not L-Arginine or L-Ornithine, Acts as a Hypothermic Agent in Chicks
Oral Administration of L-Citrulline, but not L-Arginine or L-Ornithine, Acts as a Hypothermic Agent in Chicks
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DOI：
10.2141/jpsa.0150014
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发表时间：
2015-10-01
影响因子：
1.5
通讯作者：
Furuse, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Chowdhury, Vishwajit S.;Shigemura, Asako;Furuse, Mitsuhiro
一些氨基酸是关键代谢途径的重要调节剂，并且是多种生理功能所必需的。然而，对氨基酸的体温调节功能知之甚少。因此，在本研究中，雏鸡通过中枢或经口给予L-瓜氨酸（L-Cit）、L-精氨酸（L-Arg）或L-鸟氨酸（L-Orn），以监测直肠温度的变化。在实验1中，将氨基酸（L-Cit、L-Arg和L-Orn）通过侧脑室（i. c. v.）以1 μ mol/10 μ l的剂量注射，以监测这些氨基酸在120分钟实验期间对直肠温度的影响。在实验2中，小鸡以15 mmol/10 ml/kg体重的剂量经口给予相同的氨基酸。在实验3中，鸡通过口服施用接受三个剂量的L-Cit（3.75、7.5或15 mmol/10 ml/kg体重）。静脉注射任何研究的氨基酸都不会改变体温，但口服L-Cit可显著降低体温。重要的是，最高的确实有效地降低了体温。这些结果表明，外周L-Cit具有降低雏鸡体温的作用，这可能是一个新的候选人，以减轻家禽在夏季热应激的高体温。
Some amino acids are important regulators of key metabolic pathways and necessary for several physiological functions. However, little is know about thermoregulatory functions of amino acids. In this study, therefore chicks were either centrally or orally administered with L-citrulline (L-Cit), L-arginine (L-Arg) or L-ornithine (L-Orn) to monitor changes in rectal temperature. In Experiment 1, the amino acids (L-Cit, L-Arg and L-Orn) were administered into the left ventricle of the chicks by intracerebroventricular (i.c.v.) injection at a dose of 1 mu mol/10 mu l to monitor the effects of these amino acids on rectal temperature during 120 min of the experimental period. In Experiment 2, chicks received the same amino acids by oral administration at a dose of 15 mmol/10 ml/kg body weight. In Experiment 3, chicks received three doses of L-Cit (3.75, 7.5 or 15 mmol/10 ml/kg body weight) by oral administration. I.c.v. injection with any of the amino acids studied did not alter body temperature, but oral administration of L-Cit significantly reduced body temperature. Importantly, the highest does effectively reduced body temperature. These results suggest that peripheral L-Cit has a hypothermic function in chicks, which may be a new candidate to minimize high body temperature in poultry during summer heat stress.