Comparative study using 685-nm and 830-nm lasers in the tissue repair of tenotomized tendons in the mouse
Comparative study using 685-nm and 830-nm lasers in the tissue repair of tenotomized tendons in the mouse
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DOI：
10.1089/pho.2006.24.754
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发表时间：
2006-12-01
影响因子：
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通讯作者：
Vidal, Benedito Campos
中科院分区：
文献类型：
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作者：
Carrinho, Patricia M.;Muniz Renno, Ana Claudia;Vidal, Benedito Campos
目的：探讨685 nm和830 nm激光照射对小鼠跟腱切断术后跟腱愈合过程的影响。背景资料：一些作者已经表明，低水平激光治疗（LLLT）能够加速肌腱组织损伤后的愈合过程，增加成纤维细胞增殖和胶原合成。然而，LLLT作用于愈合过程的机制尚不完全清楚。方法：48只雄性小鼠随机分为6个实验组：A组，脱毒动物，685 nm激光治疗，剂量为3 J/cm(2)；B组，脱毒动物，685 nm激光，剂量为10 J/cm(2)；C组，脱毒动物，830 nm激光，剂量为3 J/cm(2)；D组，脱毒动物，830 nm激光，剂量为10 J/cm(2)；E组，损伤对照组（安慰剂治疗）；F组为无损伤标准对照。肌腱切断后第13天处死动物，手术切除肌腱，用偏振显微镜进行定量分析，通过双折射（光学延迟[OR]）测量胶原纤维组织。结果：各治疗组的OR值均高于损伤对照组。A组（685 μ m, 3 J/cm(2)）动物胶原束的组织和聚集最好，其次是C组和B组，最后是d组。结论：本研究中使用的所有波长和影响都能有效加速跟腱切开术后的愈合过程，特别是685 nm激光照射后，3 J/cm(2)。提示存在波长、组织特异性和剂量依赖性。激光对肌腱修复作用的生理机制有待进一步研究。
Objective: The objective of this study was to evaluate the effects of 685- and 830-nm laser irradiations, at different fluences on the healing process of Achilles tendon (Tendon calcaneo) of mice after tenotomy. Background Data: Some authors have shown that low-level laser therapy (LLLT) is able to accelerate the healing process of tendinuos tissue after an injury, increasing fibroblast cell proliferation and collagen synthesis. However, the mechanism by which LLLT acts on healing process is not fully understood. Methods: Forty-eight male mice were divided into six experimental groups: group A, tenomized animals, treated with 685 nm laser, at the dosage of 3 J/cm(2); group B, tenomized animals, treated with 685-nm laser, at the dosage of 10 J/cm(2); group C, tenomized animals, treated with 830-nm laser, at dosage of 3 J/cm(2); group D, tenomized animals, treated with 830-nm laser, at the dosage of 10 J/cm(2); group E, injured control (placebo treatment); and group F, non-injured standard control. Animals were killed on day 13 post-tenotomy, and their tendons were surgically removed for a quantitative analysis using polarization microscopy, with the purpose of measuring collagen fibers organization trough the birefringence (optical retardation [OR]). Results: All treated groups showed higher values of OR when compared to injured control group. The best organization and aggregation of the collagen bundles were shown by the animals of group A (685 run, 3 J/cm(2)), followed by the animals of group C and B, and finally, the animals of group D. Conclusion: All wavelengths and fluences used in this study were efficient at accelerating the healing process of Achilles tendon post-tenotomy, particularly after the 685-nm laser irradiation, at 3 J/cm(2). It suggests the existence of wavelength tissue specificity and dose dependency. Further studies are required to investigate the physiological mechanisms responsible for the effects of laser on tendinuos repair.