Preservation of reproductive behaviors during modest cooling: rapid cold-hardening fine-tunes organismal response
Preservation of reproductive behaviors during modest cooling: rapid cold-hardening fine-tunes organismal response
复制标题
适度冷却期间繁殖行为的保存：快速冷硬化微调有机体反应
DOI：
10.1242/jeb.00951
复制
发表时间：
2004
影响因子：
2.8
通讯作者：
R. Lee
中科院分区：
文献类型：
--
作者：
Scott M. Shreve;Jonathan D. Kelty;R. Lee
摘要本研究的主要目的是确定（1）快速冷硬化（RCH）是否在适度冷却期间保留繁殖行为，（2）较低温度下交配成功率的提高是否会以较高温度下性能下降为代价，以及（3）RCH是否与代谢率升高有关。实验前，将黑腹果蝇（双翅目：果蝇科）暴露在 16°C 下 2 小时，使其迅速冷硬化。仅 7°C（23°C 至 16°C）的温度下降就阻止了一半 (11/22) 的对照黑腹果蝇进行任何求偶活动。相比之下，大多数 RCH 情侣都在求爱 (17/20)。此外，7°C 转移阻止了每对对照果蝇的交配，而超过一半 (11/20) 的 RCH 对进行了交配。当 RCH 对在 23°C 下进行测试时，没有证据表明求爱或交配性能受损。最后，RCH 显然不是一个需要能量的过程，因为在其诱导过程中没有检测到代谢率的增加。总的来说，这些数据表明 RCH 可以不断调整昆虫的生理状态，以适应环境温度的轻微变化。此外，RCH反应不仅限于低温保护和在寒冷中生存，还可以在全年中至高温下保留更微妙的行为，例如求偶。
SUMMARY The primary objectives of this study were to determine (1) whether rapid cold-hardening (RCH) preserves reproductive behaviors during modest cooling, (2) whether increased mating success at a lower temperature comes at the cost of decreased performance at a higher temperature and (3) whether RCH is associated with an elevated metabolic rate. Drosophila melanogaster (Diptera: Drosphilidae) were rapidly cold-hardened by a 2-h exposure to 16°C prior to experiments. A temperature decrease of only 7°C (23°C to 16°C) prevented half (11/22) of the control pairs of D. melanogaster from engaging in any courtship activity. By contrast, most RCH pairs courted (17/20). Additionally, the 7°C transfer prevented mating in every pair of control flies, whereas more than half (11/20) of the RCH pairs mated. There was no evidence of impaired courtship or mating performance when RCH pairs were tested at 23°C. Finally, RCH is apparently not an energy-demanding process because no increase in the metabolic rate was detected during its induction. Overall, these data demonstrate that RCH serves to constantly fine-tune an insect's physiological state to match slight changes in environmental temperature. Furthermore, the RCH response is not restricted to cryoprotection and survival in the cold but also preserves more subtle behaviors, such as courtship, at moderate to high temperatures throughout the year.