Commitment to the B lymphoid lineage occurs before DH-JH recombination
Commitment to the B lymphoid lineage occurs before DH-JH recombination
复制标题
DOI：
10.1084/jem.189.4.735
复制
发表时间：
1999-02-15
影响因子：
15.3
通讯作者：
Hardy, RR
中科院分区：
文献类型：
--
作者：
Allman, D;Li, J;Hardy, RR
B淋巴细胞生成中的谱系定型仍然知之甚少，因为无法清楚地定义新定型的B谱系祖细胞及其多能后代。我们研究了三个最近描述的祖细胞群体在成年小鼠骨髓分化成每一个造血谱系的潜力。其中最早的，称为分数（Fr）。A（0），表现出髓系、红系以及B和T淋巴祖细胞活性，包括具有髓系/B淋巴潜能的单个细胞。与此形成鲜明对比的是，两个后来的人口，被称为法国。A（1）和A（2）的特征在于表面B220表达和生殖系免疫球蛋白重链（IgH）基因座的转录，对除B淋巴细胞外的所有造血谱系缺乏祖细胞活性。这些观察结果，连同单细胞聚合酶链反应分析显示在每个群体中缺乏D-H/J（H）重排，以及当这些群体来自重组激活基因（Rag）-1（-/-）和J（H）（-/-）小鼠时显示相同前体潜力的实验，证明向B淋巴谱系的定型发生在V（H）D（H）J（H）重组之前且独立于V（H）D（H）J（H）重组。
Lineage commitment in B lymphopoiesis remains poorly understood due to the inability to clearly define newly committed B lineage progenitors and their multipotential descendants. We examined the potential of three recently described progenitor populations in adult mouse bone marrow to differentiate into each hematopoietic lineage. The earliest of these, termed fraction (Fr.) A(0), exhibited myeloid, erythroid, and B and T lymphoid progenitor activity and included individual cells with myeloid/B lymphoid potential. In sharp contrast, two later populations, termed Frs. A(1) and A(2) and characterized by surface B220 expression and transcription of the germline immunoglobulin heavy chain (IgH) locus, lacked progenitor activity for all hematopoietic lineages except B lymphocytes. These observations, together with single cell polymerase chain reaction analysis showing a lack of D-H/J(H) rearrangements in each population and experiments showing identical precursor potentials when these populations were derived from recombination activating gene (Rag)-1(-/-) and J(H)(-/-) mice, demonstrate that commitment to the B lymphoid lineage occurs before and independently of V(H)D(H)J(H) recombination.