Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules
Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules
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DOI：
10.1002/smll.200600112
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发表时间：
2006-08-01
期刊：
影响因子：
13.3
通讯作者：
Diez, Stefan
中科院分区：
文献类型：
--
作者：
Dinu, Cerasela Zoica;Opitz, Joerg;Diez, Stefan
我们开发了一种操纵双功能DNA分子的技术：一端被巯基化以结合到图案化的金表面，另一端被生物素化以结合到在驱动蛋白涂覆的表面上滑动的微管。我们发现DNA分子可以在金垫和运动的微管之间被拉伸和过度拉伸，并且它们可以形成动态网络。这是一个原理证明，生物机器可以在体外用于完成结构化DNA模板的平行形成，这将在生物物理学和纳米电子学中有应用。
We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof-of-principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.