Methanolinea mesophila sp nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov within the order Methanomicrobiales
Methanolinea mesophila sp nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov within the order Methanomicrobiales
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DOI：
10.1099/ijs.0.035048-0
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Imachi, Hiroyuki
中科院分区：
文献类型：
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作者：
Sakai, Sanae;Ehara, Masayuki;Imachi, Hiroyuki
一种新的嗜中温，氢营养产甲烷菌，命名为菌株TNRT，分离自厌氧，丙酸降解富集培养，最初建立从台湾的稻田土壤样品。细胞为非运动的杆状，长2.0-6.5 μ m，宽0.3 μ m。在指数生长后期的培养物中也观察到丝状体（最大约100 μ m）和球状体（直径约1 μ m）细胞。菌株TNRT在20-40 ℃（最佳在37 ℃）、pH 6.5-7.4（最佳在pH 7.0）和0-25 g NaCl I-1（最佳在0 g NaCl I-1）存在下生长。该菌株利用H-2/CO2和甲酸盐进行生长并产生甲烷。基因组DNA的G+C含量为56.4mol%。基于16 S rRNA基因和产甲烷菌特异性标记基因mcrA的序列，菌株TNRT与迟缓甲烷菌NOBI-1（T）最接近;序列相似性水平分别为94.8%和86.4%。16 S rRNA基因序列的相似性表明，菌株TNRT和M. tardaNOBI-1（T）代表同一属内的不同种。这是由共同的表型特性支持的，包括底物使用和细胞形态，以及生长温度的差异。基于这些遗传和表型特性，菌株TNRT被认为代表了嗜中温甲醇菌属的一个新种，并建议将其命名为嗜中温甲醇菌新种;模式菌株为TNRT（=NBRC 105659（T）= DSM 23604（T））。此外，我们还建议甲烷微生物目中E1/E2群的家族地位，并将其命名为甲烷调节科家族。nov.提出;模式属的家庭是甲烷调节。
A novel mesophilic, hydrogenotrophic methanogen, designated strain TNRT, was isolated from an anaerobic, propionate-degradation enrichment culture that was originally established from a rice field soil sample from Taiwan. Cells were non-motile rods, 2.0-6.5 mu m long by 0.3 mu m wide. Filamentous (up to about 100 mu m) and coccoid (about 1 mu m in diameter) cells were also observed in cultures in the late exponential phase of growth. Strain TNRT grew at 20-40 degrees C (optimally at 37 degrees C), at pH 6.5-7.4 (optimally at pH 7.0) and in the presence of 0-25 g NaCl I-1 (optimally at 0 g NaCl I-1). The strain utilized H-2/CO2 and formate for growth and produced methane. The G+C content of the genomic DNA was 56.4 mol%. Based on sequences of both the 16S rRNA gene and the methanogen-specific marker gene mcrA, strain TNRT was related most closely to Methanolinea tarda NOBI-1(T); levels of sequence similarities were 94.8 and 86.4%, respectively. The 16S rRNA gene sequence similarity indicates that strain TNRT and M. tarda NOBI-1(T) represent different species within the same genus. This is supported by shared phenotypic properties, including substrate usage and cell morphology, and differences in growth temperature. Based on these genetic and phenotypic properties, strain TNRT is considered to represent a novel species of the genus Methanolinea, for which the name Methanolinea mesophila sp. nov. is proposed; the type strain is TNRT (=NBRC 105659(T)= DSM 23604(T)). In addition, we also suggest family status for the E1/E2 group within the order Methanomicrobiales, for which the name Methanoregulaceae fam. nov. is proposed; the type genus of family is Methanoregula.