Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis
Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis
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DOI：
10.1016/j.joca.2006.05.008
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发表时间：
2006-12-01
影响因子：
7
通讯作者：
Masuda, K.
中科院分区：
文献类型：
--
作者：
Akeda, K.;An, H. S.;Masuda, K.
目的：富血小板血浆（PRP）是血浆中含有高水平多种生长因子的一部分。本研究的目的是研究的影响，PRP对细胞增殖和基质合成的猪软骨细胞培养在藻酸盐珠，促进保留的软骨细胞表型的条件，以确定使用这种血浆衍生的材料工程cartilage.Design：PRP和血小板贫乏的血浆（PPP）制备从成年猪的血液。将成年猪软骨细胞在10%PRP、10%PPP或10%胎牛血清（FBS）存在下培养3天。结果：10%PRP处理后，细胞DNA含量显著增加（+11%，vs FBS; P < 0.01; vs PPP; P <0.001），细胞增殖、蛋白多糖（PG）和胶原合成均明显增加（+11%，vs FBS; P <0.01）。PRP处理的软骨细胞合成PG和胶原的能力明显高于FBS或PPP处理的软骨细胞（PG; PRP：+115% vs FBS;+151% vs PPP，均P < 0.0001;胶原：+163% vs FBS;+163% vs PPP，均P < 0.0001）。生化分析表明，治疗与PRP生长因子并没有显着影响类型的PG和胶原蛋白产生的猪软骨细胞，这表明细胞表型保持稳定的存在下PRP.Conclusion：从自体血中分离的PRP可能是有用的合成代谢生长因子刺激软骨细胞工程软骨组织的来源。(C)2006年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Platelet-rich plasma (PRP) is a fraction of plasma that contains high levels of multiple growth factors. The purpose of this study was to examine the effects of PRIP on cell proliferation and matrix synthesis by porcine chondrocytes cultured in alginate beads, conditions that promote the retention of the chondrocytic phenotype, in order to determine the plausibility of using this plasma-derived material for engineering cartilage.Design: PRP and platelet-poor plasma (PPP) were prepared from adult porcine blood. Adult porcine chondrocytes were cultured in the presence of 10% PRP, 10% PPP or 10% fetal bovine serum (FBS) for 3 days. Cell proliferation, proteoglycan (PG) and collagen synthesis were quantified, and the structure of newly synthesized PG and collagen was characterized.Results: Treatment with 10% PRP resulted in a small but significant increase in DNA content (+11%, vs FBS; P < 0.01; vs PPP; P < 0.001). PG and collagen syntheses by the PRP-treated chondrocytes were markedly higher than those by chondrocytes treated by FBS or PPP (PG; PRP: +115% vs FBS; +151% vs PPP, both P < 0.0001, collagen; PRP: +163% vs FBS; +163% vs PPP, both P < 0.0001). Biochemical analyses revealed that treatment with PRP growth factors did not markedly affect the types of PGs and collagens produced by porcine chondrocytes, suggesting that the cells remained phenotypically stable in the presence of PRP.Conclusion: PRP isolated from autologous blood may be useful as a source of anabolic growth factors for stimulating chondrocytes to engineer cartilage tissue. (C) 2006 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.