BIOFLAVONOID REGULATION OF ATPASE AND HEXOKINASE ACTIVITY IN EHRLICH ASCITES CELL MITOCHONDRIA
BIOFLAVONOID REGULATION OF ATPASE AND HEXOKINASE ACTIVITY IN EHRLICH ASCITES CELL MITOCHONDRIA
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DOI：
10.1016/0005-2728(77)90221-3
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
GRAZIANI, Y
中科院分区：
文献类型：
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作者：
GRAZIANI, Y
来自艾氏腹水瘤（EA）[小鼠]细胞线粒体的线粒体ATP酶（EC 3.6.1.3）在生理浓度的ATP下被D - 葡萄糖抑制。线粒体结合的己糖激酶产生ADP似乎是D - 葡萄糖抑制作用的原因。通过一个ATP再生系统实现了ADP对EA细胞线粒体ATP酶抑制作用的逆转。线粒体结合的己糖激酶从线粒体上解离消除了D - 葡萄糖的抑制作用。己糖激酶重新结合到线粒体上使D - 葡萄糖对EA细胞线粒体ATP酶的抑制作用重新产生。生物类黄酮如槲皮素抑制线粒体己糖激酶活性，但不改变分离的EA线粒体的线粒体ATP酶活性。生物类黄酮对线粒体结合的己糖激酶活性的抑制作用可从EA线粒体上解离，并且似乎与该酶的调节位点而非催化位点相关。
The mitochondrial ATPase (EC 3.6.1.3) from Ehrlich ascites [EA] [mouse] cell mitochondria, was inhibited by D-glucose under physiological concentrations of ATP. The generation of ADP by the mitochondrial bound hexokinase, seems to be the reason for the D-glucose inhibitory effect. Reversal of the inhibitory effect of ADP on EA cell mitochondria ATPase by an ATP-regenerating system was achieved. Dissociation of mitochondrial bound hexokinase from the mitochondria eliminated the inhibitory effect of D-glucose. Rebinding of the hexokinase to the mitochondria regenerated the D-glucose inhibitory effect on EA cell mitochondria ATPase. Bioflavonoids such as quercetin inhibit the mitochondrial hexokinase activity, but do not change the mitochondrial ATPase activity of isolated EA mitochondria. The inhibitory effect of bioflavonoids on mitochondrial bound hexokinase activity is dissociable from the EA mitochondria and seems to be associated with regulatory rather than catalytic sites of the enzyme.