Characterization of the two nonidentical ArgR regulators ofTetragenococcus halophilusand their regulatory effects on arginine metabolism
Characterization of the two nonidentical ArgR regulators ofTetragenococcus halophilusand their regulatory effects on arginine metabolism
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嗜盐四联球菌两个不同的 ArgR 调节因子的表征及其对精氨酸代谢的调节作用
DOI：
10.1007/s00253-020-10868-6
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发表时间：
2020-09-03
影响因子：
5
通讯作者：
Luo, Lixin
中科院分区：
文献类型：
--
作者：
Lin, Jieting;Luo, Xiaotong;Luo, Lixin
嗜盐乳酸菌嗜盐四联球菌已广泛应用于食品的高盐发酵过程中。先前的研究表明，精氨酸的分解代谢可能有助于T的渗透胁迫适应。嗜盐杆菌。不同寻常的是，在T染色体上。嗜盐杆菌位于精氨酸脱亚胺酶 (ADI) 操纵子之前，定位两个共转录基因，均编码 ArgR 调节因子；类似的结构很少被发现，监管机构的作用也尚未得到证实。在本研究中，这两个不同的ArgR调节因子对T的精氨酸代谢的调节作用。对嗜盐杆菌进行了研究。结果表明，这两种调节因子在精氨酸代谢中发挥着不同的作用，ArgR1通过与启动子序列结合并抑制基因转录，作为ADI途径的负调节因子，添加精氨酸或高渗胁迫条件可以解除ArgR1的抑制，而ArgR2负调节参与精氨酸生物合成的基因。我们的研究发现，尽管 ArgR 调节剂的众所周知的作用是精氨酸分解代谢的激活剂和精氨酸生物合成的抑制剂，在大多数研究的细菌中发现它们具有一种 ArgR 调节剂，但 T 的两种不同的 ArgR 调节剂。嗜盐菌都充当抑制因子，当感知环境变化时，抑制就会受到调节。通过揭示精氨酸代谢的调节，当前的研究为嗜盐菌在生物技术中的未来应用提供了分子见解和潜在工具。
The halophilic lactic acid bacteriumTetragenococcus halophilushas been widely used in high-salinity fermentation processes of food. Previous studies have indicated that the catabolism of arginine may contribute to the osmotic stress adaptation ofT. halophilus. Unusually, in the chromosome ofT. halophilus, preceding the arginine deiminase (ADI) operon, locate two co-transcribed genes, both encoding an ArgR regulator; similar structure was rarely found and the roles of the regulators have not been demonstrated. In the current study, regulatory roles of these two nonidentical ArgR regulators on the arginine metabolism ofT. halophiluswere investigated. The results show that these two regulators play different roles in arginine metabolism, ArgR1 acts as a negative regulator of the ADI pathway by binding to the promoter sequences and repressing the transcription of genes, and the addition of arginine or hyper-osmotic stress conditions can abolish the ArgR1 repression, whereas ArgR2 negatively regulates the genes involved in arginine biosynthesis. Our study found that despite the commonly known roles of the ArgR regulators as the activator of arginine catabolism and the repressor of arginine biosynthesis, which are found in most studied bacteria possessed one ArgR regulator, the two nonidentical ArgR regulators ofT. halophilusboth act as repressors, and the repression by which is regulated when sensing changes of environments. By revealing the regulation of arginine metabolism, the current study provides molecular insights and potential tools for future applications of halophiles in biotechnology.