Biosensor for determining average iron content of ferritin by measuring its optical dispersion
Biosensor for determining average iron content of ferritin by measuring its optical dispersion
复制标题
通过测量铁蛋白的光学色散来确定铁蛋白平均铁含量的生物传感器
DOI：
10.1117/12.2542162
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Gupta R
中科院分区：
文献类型：
--
作者：
Gupta R
铁蛋白的平均铁含量有可能作为早期识别高危创伤患者的生物标志物。然后可以给予适当的治疗以降低发病率和死亡率。目前，蛋白质和铁水平分别使用酶联免疫吸附测定法（ELISA）和紫外或原子吸收光谱法（AAS）分别测量，但使用两种完全不同的方法增加了组合测量的复杂性和分析时间。因此，这些方法不适合于热分析。为了解决这一差距，我们报告了一种生物传感器，用于测量铁蛋白的平均铁含量在一个单一的步骤。该生物传感器是基于染料掺杂的漏波导（LW），其在整个可见光波长范围内工作，因此允许测量铁蛋白和脱铁铁蛋白的光学色散的差异，以确定蛋白质的平均铁含量。LW生物传感器包括1.54微米厚的中孔壳聚糖平板波导，其具有针对铁蛋白/脱铁铁蛋白的固定化抗体，以测量110 nM蛋白质的光学色散。基于基线噪声，该方法的铁蛋白/脱铁铁蛋白的检测限约为700 pM。该生物传感器具有显著的潜力，可用于血清铁蛋白平均铁含量的快速测量，并在未来，总蛋白浓度。
Average iron content of ferritin has a potential to serve as a biomarker for early identification of high-risk trauma patients at point-of-care (PoC). Appropriate therapies can then be administered to reduce morbidity and mortality. Currently, protein and iron levels are measured separately using enzyme-linked immunosorbent assay (ELISA) and UV or atomic absorption spectroscopy (AAS) respectively, but the use of two completely different methods adds to the complexity and analysis time of the combined measurement. As a result, these methods are unsuitable for PoC analysis. To address this gap, we report a biosensor for measuring the average iron content of ferritin in a single step. The biosensor was based on a dye-doped leaky waveguide (LW), which operates in the entire visible wavelength range, and hence allowed the measurement of differences in the optical dispersion of ferritin and apoferritin to determine the average iron content of the protein. The LW biosensor comprised a 1.54 micron thick mesoporous chitosan slab waveguide with immobilized antibodies against ferritin/ apoferritin to measure the optical dispersion of 110 nM protein. Based on the baseline noise, the limit of detection for this method is ~700 pM for ferritin/ apoferritin. The biosensor has a significant potential for PoC measurement of the average iron content of serum ferritin and, in future, the total protein cencentration.
影响因子：
4.1
作者：
R. Crichton;R. Eason;A. Barclay;C. Bryce
通讯作者：
C. Bryce
铁蛋白的线性和非线性磁光。
DOI：
10.1063/1.3159844
发表时间：
2009
期刊：
The Journal of chemical physics
影响因子：
--
作者：
M. Pankowska;Andrzej Dobek
通讯作者：
Andrzej Dobek
影响因子：
3.4
作者：
Kell, Douglas B.;Pretorius, Etheresia
通讯作者：
Pretorius, Etheresia