Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells.
Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells.
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DOI：
10.1038/s41597-022-01846-w
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发表时间：
2022-11-29
期刊：
影响因子：
9.8
通讯作者：
Fukuda, Tomokazu
中科院分区：
文献类型：
--
作者：
Matsusaka, Himari;Wu, Tao;Furuya, Kai;Yamada-Kato, Tomoe;Bai, Lanlan;Tomita, Hiroshi;Sugano, Eriko;Ozaki, Taku;Kiyono, Tohru;Okunishi, Isao;Fukuda, Tomokazu
与睾酮相关的类固醇激素与各种疾病有关，包括前列腺癌和雄激素性脱发(AGA)。睾酮或二羟基睾酮(DHT)在血液中循环，与细胞质中的雄激素受体(AR)结合，最后进入细胞核激活下游靶基因。我们先前发现永生化的毛乳头细胞(DPC)失去了AR的表达，这可能是由于DPC的细胞反复传代所致。为了补偿AR的表达，建立了外源表达AR的DPC。在这项研究中，我们对表达AR和不表达AR的DPC在DHT存在或不存在的情况下进行了RNA-Seq分析，以确定受AR信号调节的下游靶基因。此外，我们用硫酸米诺地尔治疗DPC，这有可能治疗AGA。这是第一次对参与DPC中的睾酮信号传递的下游基因进行全面分析。我们的手稿为发现前列腺癌和AGA的分子靶点提供了高优先级的数据。
Testosterone-related steroid hormones are associated with various types of diseases, including prostate cancer and androgenetic alopecia (AGA). The testosterone or dihydroxy testosterone (DHT) circulates through the blood, binds to the androgen receptor (AR) in the cytoplasm, and finally enters the nucleus to activate downstream target genes. We previously found that immortalized dermal papilla cells (DPCs) lost AR expression, which may be explained by the repeated cell passages of DPCs. To compensate for the AR expression, DPCs that express AR exogenously were established. In this study, we performed an RNA-Seq analysis of the AR-expressing and non-AR-expressing DPCs in the presence or absence of DHT to identify the downstream target genes regulated by AR signalling. Furthermore, we treated DPCs with minoxidil sulphate, which has the potential to treat AGA. This is the first comprehensive analysis to identify the downstream genes involved in testosterone signalling in DPCs. Our manuscript provides high-priority data for the discovery of molecular targets for prostate cancer and AGA.
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