Identification of noradrenergic nerve terminals immunoreactive for neuropeptide Y and vasoactive intestinal peptide in the rat kidney.
Identification of noradrenergic nerve terminals immunoreactive for neuropeptide Y and vasoactive intestinal peptide in the rat kidney.
复制标题
大鼠肾脏中对神经肽 Y 和血管活性肠肽具有免疫反应性的去甲肾上腺素能神经末梢的鉴定。
DOI：
10.1002/aja.1001840303
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Beal,JA
中科院分区：
文献类型：
--
作者：
Knight,DS;Fabre,RD;Beal,JA
对大鼠肾脏的冷冻切片和振动切片进行单标记或双标记，以观察免疫反应性酪氨酸羟化酶（THI）、多巴胺β羟化酶（DBHI）、血管活性肠肽（VIPI）和神经肽Y（NPYI）。将大鼠用含或不含0.15%苦味酸的2 - 4%多聚甲醛灌注固定，并在缓冲液中冲洗18 - 48 h。在振动切片机切片中用辣根过氧化物酶标记单个抗原，而在同一组织切片中使用低温恒温器切片用过氧化物酶标记一种抗原，用荧光团标记另一种抗原。DBHI去甲肾上腺素能神经的密集丛支配肾动脉树，并且这些神经也支配叶间静脉和肾盏。免疫反应性NPY共定位于大多数这些神经中，但一些肾内去甲肾上腺素能神经不含NPY，但含有VIP免疫反应性。NPYI神经的分布类似于DBHI神经，而大多数血管周围的VIP免疫反应的去甲肾上腺素能神经支配选定的弓状动脉和小叶间动脉。少数非肾上腺素能VIPI神经支配肾盏。
Cryostat‐and vibratome‐cut sections of rat kidneys were singly or doubly labeled to visualize immunoreactive tyrosine hydroxylase (THI), dopamine beta‐hydroxylase (DBHI), vasoactive intestinal peptide (VIPI), and neuropeptide Y (NPYI). Rats were perfusion fixed with 2‐4% paraformaldehyde with or without 0.15% picric acid and rinsed in buffer for 18‐48 hr. Single antigens were labeled with horseradish per‐oxidase in vibratome sections, whereas cryostat sections were used to label one antigen with perox‐idase and another with a fluorophore in the same tissue section. A dense plexus of DBHI noradren‐ergic nerves innervates the renal arterial tree, and such nerves innervate the interlobar veins and renal calyx as well. Immunoreactive NPY is colocal‐ized in most of these nerves, but some intrarenal noradrenergic nerves do not contain NPY but do contain VIP immunoreactivity. The distribution of NPYI nerves resembles that of DBHI nerves, whereas most perivascular noradrenergic nerves immunoreactive for VIP innervate selected arcuate and interlobular arteries. A small population of nonadrenergic, VIPI nerves innervates the renal calyx.