Effect of bis(maltolato) oxovanadium on experimental vascular endothelial dysfunction.
Effect of bis(maltolato) oxovanadium on experimental vascular endothelial dysfunction.
复制标题
双（麦芽糖）氧钒对实验性血管内皮功能障碍的影响。
DOI：
10.1007/s00210-006-0066-1
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Singh,Manjeet
中科院分区：
文献类型：
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作者：
Shah,DhvanitI;Singh,Manjeet
本研究旨在探讨蛋白酪氨酸磷酸酶抑制剂双麦芽氧钒（BMOV）对高胆固醇血症和高血压诱导的血管内皮功能障碍的影响。高脂饮食（8周）和醋酸脱氧皮质酮（DOCA; 40 mg kg − 1，s.c.）分别给药于大鼠以产生高胆固醇血症和高血压（平均动脉血压> 120 mmHg）。采用离体主动脉环制备、胸主动脉电子显微镜检查和血清亚硝酸盐/硝酸盐浓度评估血管内皮功能障碍。血清硫代巴比妥酸反应物质（TBARS）估计氧化应激。BMOV（0.2 mg/ml饮用水）或阿托伐他汀（30 mg kg − 1，p.o.）显着改善乙酰胆碱诱发的内皮依赖性舒张，血管内皮衬里，血清亚硝酸盐/硝酸盐浓度，血清TBARS在高胆固醇血症和高血压大鼠。然而，BMOV的这种改善作用已被L-NAME（25 mg kg − 1，i.p.）阻止，NOS抑制剂，或格列本脲（5 mg kg − 1，腹腔注射），ATP敏感性K+通道的阻断剂。因此，可以得出结论，BMOV诱导的PTK抑制可以改善血管内皮功能障碍。
The study has been designed to investigate the effect of bis(maltolato) oxovanadium (BMOV), a protein tyrosine phosphatase inhibitor, on hypercholesterolemia and hypertension-induced vascular endothelial dysfunction. High fat diet (8 weeks) and deoxycorticosterone acetate (DOCA; 40 mg kg−1, s.c.) were administered to rats to produce hypercholesterolemia and hypertension (mean arterial blood pressure >120 mmHg) respectively. Vascular endothelial dysfunction was assessed using isolated aortic ring preparation, electron microscopy of thoracic aorta, and serum concentration of nitrite/nitrate. Serum thiobarbituric acid reactive substances (TBARS) were estimated to assess oxidative stress. BMOV (0.2 mg/ml in drinking water) or atorvastatin (30 mg kg−1, p.o.) markedly improved acetylcholine-evoked endothelium-dependent relaxation, lining of vascular endothelium, serum nitrite/nitrate concentration, and serum TBARS in hypercholesterolemic and hypertensive rats. However, this ameliorative effect of BMOV has been prevented by L-NAME (25 mg kg−1, i.p.), an inhibitor of NOS, or by glibenclamide (5 mg kg−1, i.p.), a blocker of ATP-sensitive K+channels. It may be concluded that BMOV-induced inhibition of PTPase may improve vascular endothelial dysfunction.