Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis
Molecular Characterization of the Cytoplasmic Interacting Protein of the Receptor Kinase IRK Expressed in the Inflorescence and Root Apices of Arabidopsis
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DOI：
10.1271/bbb.68.2598
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发表时间：
2004-01
期刊：
影响因子：
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通讯作者：
Jun-ichiro Hattan;H. Kanamoto;M. Takemura;A. Yokota;T. Kohchi
中科院分区：
文献类型：
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作者：
Jun-ichiro Hattan;H. Kanamoto;M. Takemura;A. Yokota;T. Kohchi
在植物中，分生组织的维持和分化是通过受体样激酶（RLK）进行细胞间通讯来调节的，但RLK信号传导的潜在分子机制在很大程度上仍然未知。利用酵母双杂交技术鉴定了一个拟南芥花序和根尖受体样激酶（IRK）的胞质相互作用蛋白，命名为IRK-interacting protein（IRKI）。IRKI是高等植物中发现的一种新的高度保守的蛋白质。IRK和IRKI之间的相互作用通过体外下拉试验证实，并通过它们在分生组织中活跃分裂细胞中的同时表达得到支持。在根尖中，IRKI的表达和定位可视化的绿色荧光蛋白（GFP）中观察到的静止中心，初始细胞，和未成熟的中柱细胞。外源生长素处理扩大了IRKI的表达，极性生长素运输抑制剂处理抑制了IRKI的表达。
Meristem maintenance and differentiation is regulated by intercellular communication through receptor-like kinases (RLKs) in plants, but the underlying molecular mechanisms of RLK signaling remain largely unknown. A cytoplasmic interactor for inflorescence and root apices receptor-like kinase (IRK), which is a typical meristematic RLK with leucine-rich repeats in Arabidopsis, was identified using a yeast two-hybrid assay and named IRK-interacting protein (IRKI). IRKI is a novel but highly conserved protein found in higher plants. The interaction between IRK and IRKI was confirmed by an in vitro pull-down assay and supported by their simultaneous expression in actively dividing cells in meristems. In the root tip, IRKI expression and localization visualized by green fluorescence protein (GFP) were observed in the quiescent center, initial cells, and immature stele cells. IRKI expression was expanded by exogenous auxin treatment and repressed by inhibitor treatment of polar auxin transport.