The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis
The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis
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DOI：
10.1074/jbc.274.37.25963
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发表时间：
1999-09-10
影响因子：
4.8
通讯作者：
Snyder, SH
中科院分区：
文献类型：
--
作者：
Lai, MM;Hong, JJ;Snyder, SH
突触囊泡的胞吐是钙依赖性的，突触结合蛋白作为钙传感器。突触囊泡的内吞作用也被假定为一个钙依赖的过程，然而，内吞钙传感器尚未发现。我们现在报告的钙依赖性磷酸酶钙调神经磷酸酶和发动蛋白1，突触内吞机制的一个组成部分之间的物理关联。钙调神经磷酸酶-发动蛋白1相互作用是钙依赖性的，对钙的EC 50在0.1-0.4 μ M范围内。钙调神经磷酸酶-发动蛋白1相互作用的破坏抑制网格蛋白介导的内吞作用。因此，钙调神经磷酸酶-发动蛋白1复合物的钙依赖性形成，传递到其他内吞外壳蛋白，提供了促进内吞作用的钙敏感机制。
Exocytosis of synaptic vesicles is calcium-dependent, with synaptotagmin serving as the calcium sensor. Endocytosis of synaptic vesicles has also been postulated as a calcium-dependent process; however, an endocytic calcium sensor has not been found. We now report a physical association between the calcium-dependent phosphatase calcineurin and dynamin 1, a component of the synaptic endocytic machinery. The calcineurin-dynamin 1 interaction is calcium-dependent, with an EC50 for calcium in the range of 0.1-0.4 mu M. Disruption of the calcineurin-dynamin 1 interaction inhibits clathrin-mediated endocytosis. Thus, the calcium-dependent formation of the calcineurin-dynamin 1 complex, delivered to the other endocytic coat proteins, provides a calcium-sensing mechanism that facilitates endocytosis.