Ultrasonic extraction, antioxidant and anticancer activities of novel polysaccharides from Chuanxiong rhizome
Ultrasonic extraction, antioxidant and anticancer activities of novel polysaccharides from Chuanxiong rhizome
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川芎新型多糖的超声提取及其抗氧化及抗癌活性
DOI：
10.1016/j.ijbiomac.2015.12.046
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发表时间：
2016-04-01
影响因子：
8.2
通讯作者：
Chen, Meiwan
中科院分区：
文献类型：
--
作者：
Hu, Jie;Jia, Xuejing;Chen, Meiwan
采用超声辅助提取技术制备川芎多糖。采用单因素试验和正交试验设计对提取条件进行优化。结果表明，最佳提取工艺条件为：超声温度80℃，超声时间40min，料水比30mL/g，用DEAE-52纤维素柱层析和Sephadex G-100柱层析分离纯化得到3个新的多糖组分：LCX0、LCX1和LCX2。分别用凝胶渗透色谱(GPC)和高效液相色谱(HPLC法)分析了三种LCX多糖组分的相对分子质量和单糖组成。并对其抗氧化活性和体外抗癌活性进行了研究。与LCX0相比，LCX2和LCX1具有较高的抗氧化活性和对HepG2、SMMC7721、A549和HCT-116细胞的生长抑制活性。因此，从川芎根茎中分离得到的多糖可能是一种具有生物医学应用前景的生物活性大分子。(C)2016年，由爱思唯尔出版。
Ultrasonic-assisted extraction technology was employed to prepare Ligusticum chuanxiong Hort polysaccharide. Single factor test and orthogonal experimental design were used to optimize the extraction conditions. The results showed that the optimal extraction conditions consisted of ultrasonic temperature of 80 degrees C, ultrasonic time of 40 min and water to raw material ratio of 30 mL/g. Three novel polysaccharides fractions, LCX0, LCX1 and LCX2, were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of three LCX polysaccharides fractions were analyzed with gel permeation chromatography (GPC) and HPLC analysis, respectively. Furthermore, the antioxidant and in vitro anticancer activities of the polysaccharides were investigated. Compared with LCX0, LCX2 and LCX1 showed relative higher antioxidant activity and inhibitory activity to the growth of HepG2, SMMC7721, A549 and HCT-116 cells. It is suggested that the novel polysaccharides from rhizome of L. chuanxiong could be promising bioactive macromolecules for biomedical use. (C) 2016 Published by Elsevier B.V.