MOLECULAR PHYLOGENY OF CERTAIN THAI GASTROCOPTINE MICRO LAND SNAILS (STYLOMMATOPHORA: PUPILLIDAE) INFERRED FROM MITOCHONDRIAL AND NUCLEAR RIBOSOMAL DNA SEQUENCES
MOLECULAR PHYLOGENY OF CERTAIN THAI GASTROCOPTINE MICRO LAND SNAILS (STYLOMMATOPHORA: PUPILLIDAE) INFERRED FROM MITOCHONDRIAL AND NUCLEAR RIBOSOMAL DNA SEQUENCES
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从线粒体和核核糖体 DNA 序列推断某些泰国胃足蜗牛（花柱纲：瞳孔螺科）的分子系统发育
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发表时间：
2004
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通讯作者：
D. O. Foighil
中科院分区：
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作者：
P. Tongkerd;Taehwan Lee;S. Panha;J. Burch;D. O. Foighil
东南亚腹足小陆螺的分类几乎完全基于壳的形态特征，侧重于孔道屏障。我们的目的是通过构建基于核(28S RDNA)和线粒体(16S RDNA)核糖体基因片段的分子系统学来测试这些特征的系统发育作用，以横断面的泰国胃粘膜多样性(15个物种)。尽管我们的分子数据集具有高度的遗传分化和同源性的特点，但它为胃肠道的进化和系统学提供了许多新的见解。三个属的标称同种与重复标本(Gyliotrachela，Hypselostoma和Anucchen)占据了树状空间的连续部分；然而，所有这三个属都是异种。在孔板中遇到了两个减少损失的例子：Sulacspira smaesarsis牢牢地嵌套在一个原本唯一的Gyliotrachela顶端分支内；落叶栖息的Hypselostoma panhai表现出与其地理上最近的岩石栖息的姊妹分类群H.Erawan显著的甲壳分化。我们的结果警告说，毫无疑问，使用孔齿特征作为诊断类属特征是不够的，这意味着生态转变可以导致快速的形态变化，并表明需要对岩石和落叶血统进行全面采样，以充分充实区域小孔小蜗牛之间的系统发育关系。我们的发现也强调了地理上相近的腹板分类群在建立当地特有物种的姐妹关系方面的作用，而不考虑表面上的形态相似性。然而，并不是所有的泰国腹科动物都有本地化的活动范围；MaehongsonKrobylos显然经历了地理上广泛的基因流动和定居模式。
The taxonomy of southeast Asian gastrocoptine pupillid micro land snails is based almost entirely on shell morphological characters, with an emphasis on apertural barriers. We aimed to test the phylogenetic utility of these characters by constructing a molecular phylogeny, based on nuclear (28S rDNA) and mitochondrial (16S rDNA) ribosomal gene fragments, for a cross-section of Thai gastrocoptine diversity (15 species). Although characterized by high levels of genetic differentiation and homoplasy, our molecular dataset provided a number of novel insights into gastrocoptine evolution and systematics. Nominal conspecifics of three genera with replicate samples (Gyliotrachela, Hypselostoma and Anauchen) occupied contiguous sections of treespace; however, all three were paraphyletic. Two inferred examples of reductive loss in apertural lamellae were encountered: Aulacospira smaesarensis was firmly nested within an otherwise exclusively Gyliotrachela tip clade; the leaf-litter-dwelling Hypselostoma panhai exhibited striking conchological differentiation from its geographically proximate rock-dwelling sister taxon H. erawan. Our results caution against the unquestioned use of apertural dentition characteristics as diagnostic generic characters, imply that ecological transitions can lead to rapid morphological change, and suggest that a comprehensive sampling of both rock and leaf-litter lineages is required to fully flesh out phylogenetic relationships among regional pupillid microsnails. Our findings also emphasize the utility of geographically proximate gastrocoptine taxa to establishing sister relationships for locally endemic species, irrespective of apparent morphological similarity. However, not all Thai gastrocoptines have localized ranges; Krobylos maehongsonensis has apparently experienced geographically extensive patterns of gene flow and colonization.