Apoptotic gene expression by human periodontal ligament cells following cyclic stretch
Apoptotic gene expression by human periodontal ligament cells following cyclic stretch
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循环拉伸后人牙周膜细胞凋亡基因的表达
DOI：
10.1111/j.1600-0765.2011.01397.x
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发表时间：
2011-12-01
影响因子：
3.5
通讯作者：
Zhang, F.
中科院分区：
文献类型：
--
作者：
Xu, C.;Hao, Y.;Zhang, F.
背景与目的：牙周膜细胞在咀嚼或正畸力引起的机械力负荷下维持牙周组织的动态平衡起着重要作用。以前的研究揭示了力驱动的牙周膜细胞通过细胞凋亡而死亡，但分配给凋亡途径的力敏感基因还没有完全被描述。本研究旨在探索力敏感基因在牙周膜细胞中的表达。材料与方法：将人牙周膜细胞暴露于20%牵张6或24 h后，应用实时荧光定量PCR芯片技术对84个与细胞凋亡有关的基因进行定量分析。结果：拉伸6 h和24 h后，分别有10个和11个基因表达上调，其中2个基因表达下调。这些基因包括肿瘤坏死因子配体家族(TNFSF8)、肿瘤坏死因子受体家族(Fas、TNFRSF10B、TNFRSF11B、TNFRSF25和CD27)、Bcl-2家族(BAG3、BAK1、BCL2L11和BCLAF1)、caspase家族(CASP5和CASP7)、凋亡抑制蛋白家族(BIRC3、BIRC6和NAIP)、caspase招募结构域家族(RIPK2和PYCARD)和死亡结构域家族(DAPK1)。结论：本研究发现了几个与牙周膜细胞凋亡途径有关的力敏感基因，为进一步研究力驱动的细胞凋亡提供了可能的靶基因。
Background and Objective: Periodontal ligament cells play an important role in maintaining homeostasis of periodontal tissue upon mechanical force loading caused by mastication or orthodontic force. Previous studies revealed force-driven periodontal ligament cell death via apoptosis, but the force-sensing genes assigned to the apoptotic pathway have not been fully characterized. The present study aimed to identify force-sensing genes implicated in the apoptotic pathway in periodontal ligament cells.Material and Methods: Human periodontal ligament cells were exposed to 20% stretch strain for 6 or 24 h, and the differential expression of 84 genes implicated in the apoptotic pathway were quantified by real-time PCR array technology.Results: Ten and 11 genes showed upregulated expression after 6 and 24 h stretches, respectively, and there were two downregulated genes in response to both 6 and 24 h stretches. These genes included those encoding the tumor necrosis factor ligand family (TNFSF8), tumor necrosis factor receptor family (FAS, TNFRSF10B, TNFRSF11B, TNFRSF25 and CD27), the Bcl-2 family (BAG3, BAK1, BCL2L11 and BCLAF1), the caspase family (CASP5 and CASP7), the inhibitor of apoptosis proteins family (BIRC3, BIRC6 and NAIP), the caspase recruitment domain family (RIPK2 and PYCARD) and the death domain family (DAPK1), as well as an oncogene (BRAF).Conclusion: This study identified several force-sensing genes implicated in the apoptotic pathway in periodontal ligament cells and should facilitate future studies on force-driven apoptosis by providing putative target genes.